β-carotene 15,15′-dioxygenase activity and cellular retinol-binding protein type II level are enhanced by dietary unsaturated triacylglycerols in rat intestines
β-carotene 15,15′-dioxygenase activity and cellular retinol-binding protein type II level are enhanced by dietary unsaturated triacylglycerols in rat intestines
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DOI：
10.1093/jn/128.10.1614
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发表时间：
1998-10-01
影响因子：
4.2
通讯作者：
Terao, J
中科院分区：
文献类型：
--
作者：
During, A;Nagao, A;Terao, J
本研究的目的是检查膳食三酰甘油对大鼠 β-胡萝卜素 15,15'-双加氧酶 (EC 1.13.11.21) 活性和细胞视黄醇结合蛋白 [CRBP (II)] 的影响。六组 8 只大鼠（7 周龄）喂食以下饮食之一：标准（STD；2.5% 大豆油）、饱和（SFA；15% 氢化大豆油）、单不饱和（MUFA；15% 橄榄油）、多不饱和（PUFA；15% 大豆油）或安妥明（CLF；2.5% 大豆油 + 0.2% 安妥明），持续 3 周。高脂饮食组MUFA和PUFA组肠道匀浆双加氧酶比活性是低脂饮食STD组的2.4倍（P < 0.01），而SFA和CLF组与STD组无显着差异。 PUFA组肠道CRBP（II）水平是STD组的1.3倍（P < 0.05），而其他组之间没有显着差异。在第二个实验中，在喂食 STD 和 PUFA 饮食的 3 周内，对大鼠肠道的双加氧酶活性进行了跟踪。食用PUFA饮食1天后，活性增强至基线水平的2.7倍，此后一直保持在较高水平，而STD组的活性则保持在较低的基线水平。因此，膳食多不饱和三酰甘油可增强大鼠肠道中 β-胡萝卜素 15,15'-双加氧酶活性和 CRBP (II) 水平。这些结果表明双加氧酶和 CRBP (II) 受涉及长链脂肪酸及其代谢物的相同机制调节。
The purpose of this study was to examine effects of dietary triacylglycerols on beta-carotene 15,15'-dioxygenase (EC 1.13.11.21) activity and cellular retinol-binding protein [CRBP (II)] in rats. Six groups of eight rats (7-wk old) were fed one of the following diets: standard (STD; 2.5% soybean oil), saturated (SFA; 15% hydrogenated soybean oil), monounsaturated (MUFA; 15% olive oil), polyunsaturated (PUFA; 15% soybean oil) or clofibrate (CLF; 2.5% soybean oil + 0.2% clofibrate) for 3 wk. The dioxygenase specific activities of the intestinal homogenates in the MUFA and PUFA groups fed the high fat diets were 2.4 times that of the STD group fed a law fat diet (P < 0.01), whereas the activities of the SFA and CLF groups were not significantly different from that of the STD group. The level of CRBP (II) in the intestine of the PUFA group was 1.3-fold that of the STD group (P < 0.05), whereas there were no significant differences among the other groups. In a second experiment, the dioxygenase activity of rat intestine was followed over 3 wk of feeding the STD and PUFA diets. After the PUFA diet was consumed for 1 d, the activity was enhanced to 2.7 times the baseline level and remained thereafter at that high level, whereas the activity of the STD group remained at the low baseline level. Thus, dietary polyunsaturated triacylglycerols enhanced both beta-carotene 15,15'-dioxygenase activity and CRBP (II) level in rat intestine. These results suggest that the dioxygenase and CRBP (II) are regulated by the same mechanism involving long-chain fatty acids and their metabolites.