Nicotine Analysis in Several Non-Tobacco Plant Materials
Nicotine Analysis in Several Non-Tobacco Plant Materials
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DOI：
10.1515/cttr-2016-0008
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发表时间：
2016-04-01
期刊：
影响因子：
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通讯作者：
Lawson, Darlene M.
中科院分区：
文献类型：
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作者：
Moldoveanu, Serban C.;Scott, Wayne A.;Lawson, Darlene M.
本研究描述了测定各种植物样品中的尼古丁与这种化合物的含量低。尼古丁天然存在于茄科植物中。烟草属植物含有大量尼古丁。然而，在用作食物的茄属植物（包括马铃薯、番茄、茄子）和辣椒属植物中仅存在低水平的氯。由于这些材料中尼古丁的含量在十亿分之几的范围内，因此测量很困难，并且不同研究的结果也有很大差异。本研究评估了茄科（不包括烟草属）的一些植物（果实、根、叶、块茎）和其他几种常用的食品蔬菜中的尼古丁水平。分析包括在70 ℃下用5%NaOH水溶液处理植物材料30分钟，然后用含有d（3）-尼古丁作为内标的TBME提取。在7890 B/7000 C GC-MS/MS系统上分析TBME有机层，该系统具有30 m x 0.25 mm，0.25 m薄膜CAM柱。MS/MS系统在MRM正离子化模式下工作，监测尼古丁的跃迁162 - 84和d（3）-尼古丁的跃迁165 - 87。特别注意保持植物材料中尼古丁的完整水平。按原样分析植物材料，不干燥，并且最小程度地暴露于污染物。单独地，测量植物材料的水分以报告基于干重的尼古丁水平。番茄和茄子（水果）的尼古丁水平约为180 ng/g干物质，绿色辣椒和马铃薯的尼古丁水平较低。在番茄叶片中检测到与番茄果实中相似的水平。来自其他植物科的材料也显示出尼古丁的痕迹。
Present study describes the determination of nicotine in various plant samples with a low content of this compound. Nicotine is found naturally in plants from the Solanaceae family The plants from Nicotiana genus contain large levels of nicotine. However, only low levels are present in plants from Solanum genus including potato, tomato, eggplant, and from Capsicum genus, which are used as food. Because the levels of nicotine in these materials are in the range of parts per billion, the measurements are difficult and the results are very different from study to study. The present study evaluated the level of nicotine in a number of plants (fruits, roots, leaves, tubers) from Solanaceae family (not including Nicotiana genus) and from several other vegetables commonly used as food. The analysis consisted of the treatment of plant material with an aqueous solution 5% NaOH at 70 degrees C for 30 min, followed by extraction with TBME containing d(3)-nicotine as an internal standard. The TBME organic layer was analyzed on a 7890B/7000C GC-MS/MS system with a 30 m x 0.25 mm, 0.25 m film CAM column. The MS/MS system worked in MRM positive ionization mode monitoring the transition 162 - 84 for nicotine and 165 - 87 for d(3)-nicotine. Particular attention was given to the preservation of the intact levels of nicotine in the plant material. The plant material was analyzed as is, without drying and with minimal exposure to contaminations. Separately, the moisture of the plant material was measured in order to report the nicotine level on a dry-basis. Levels of nicotine around 180 ng/g dry material were obtained for tomatoes and eggplant (fruit) and lower levels were obtained for green pepper and potato. Similar levels to that in the tomato fruit were detected in tomato leaves. Materials from other plant families also showed traces of nicotine.