VACUOLAR ATPASE INACTIVATION BLOCKS RECYCLING TO THE TRANS-GOLGI NETWORK FROM THE PLASMA-MEMBRANE
VACUOLAR ATPASE INACTIVATION BLOCKS RECYCLING TO THE TRANS-GOLGI NETWORK FROM THE PLASMA-MEMBRANE
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DOI：
10.1016/0014-5793(94)00437-4
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发表时间：
1994-05-23
期刊：
影响因子：
3.5
通讯作者：
BANTING, G
中科院分区：
文献类型：
--
作者：
REAVES, B;BANTING, G
TGN38/41是一种完整的膜蛋白，在跨高尔基网络(TGN)和细胞表面之间循环，但在稳态时主要定位于大鼠(NRK)细胞的TGN中。作为研究TGN和细胞表面之间循环的机制和途径的一部分，我们使用氯喹或巴菲尔霉素A(1)来调节内吞细胞器的管腔pH。我们提供的数据表明，维持内吞细胞器管腔内酸性环境的质子泵失活导致TGN38/41在早期内吞体内积累。这些数据证实了TGN38/41在质膜和TGN之间循环的观察结果，并确定了该循环途径中的一个特定块。
TGN38/41 is an integral membrane protein which recycles between the trans-Golgi network (TGN) and the cell surface but is predominantly located in the TGN of rat (NRK) cells at steady state. As part of our studies on the mechanism and route of recycling between the TGN and the cell surface we have used chloroquine or Bafilomycin A(1) to modulate the lumenal pH of endocytic organelles. The data we present demonstrate that inactivation of the proton pump which maintains the acidic environment within the lumen of endocytic organelles leads to an accumulation of TGN38/41 in early endosomes. These data confirm the observation that TGN38/41 recycles between the plasma membrane and the TGN and identifies a specific block in that recycling pathway.