Genetic Dissection of Verticillium Wilt Resistance Mediated by Tomato Ve1
Genetic Dissection of Verticillium Wilt Resistance Mediated by Tomato Ve1
复制标题
DOI：
10.1104/pp.109.136762
复制
发表时间：
2009-05-01
期刊：
影响因子：
7.4
通讯作者：
Thomma, Bart P. H. J.
中科院分区：
文献类型：
--
作者：
Fradin, Emilie F.;Zhang, Zhao;Thomma, Bart P. H. J.
由土传病原菌引起的维管束枯萎病是世界上最具破坏性的植物病害之一。黄萎病属包括寄主范围广泛的维管萎缩病病原菌。虽然长孢弧菌侵染十字花科的各种寄主，但大丽弧菌和阿尔博弧菌在包括重要经济作物在内的200多种双子叶植物上引起维管萎缩病。仅从番茄(Solanum Lycopsicum)中克隆了一个对大丽弧菌(V.dahliae)和黑曲霉(V.Albo-Atrum)1号生理小种产生抗性的基因座。该基因座被称为Ve，由两个紧密相连的反向定向基因Ve1和Ve2组成，它们编码细胞外富含亮氨酸重复序列的受体蛋白类抗病蛋白的细胞表面受体蛋白。在这里，我们证明了Ve1，而不是Ve2，在番茄中对大丽弧菌和Albo-Atrum小种1菌株产生了抗性，而对2号小种菌株没有抗性。利用病毒诱导的番茄基因沉默，Ve1下游的信号级联被证明同时需要EDS1和NDR1。此外，NRC1、ACIF、MEK2和SERK3/BAK1也是番茄Ve1的正调控基因。综上所述，Ve1介导的抗性信号与由Cf蛋白介导的抗性信号只有部分重叠，Cf蛋白是抗性蛋白中受体样蛋白的类型成员。
Vascular wilt diseases caused by soil-borne pathogens are among the most devastating plant diseases worldwide. The Verticillium genus includes vascular wilt pathogens with a wide host range. Although V. longisporum infects various hosts belonging to the Cruciferaceae, V. dahliae and V. albo-atrum cause vascular wilt diseases in over 200 dicotyledonous species, including economically important crops. A locus responsible for resistance against race 1 strains of V. dahliae and V. albo-atrum has been cloned from tomato (Solanum lycopersicum) only. This locus, known as Ve, comprises two closely linked inversely oriented genes, Ve1 and Ve2, that encode cell surface receptor proteins of the extracellular leucine-rich repeat receptor-like protein class of disease resistance proteins. Here, we show that Ve1, but not Ve2, provides resistance in tomato against race 1 strains of V. dahliae and V. albo-atrum and not against race 2 strains. Using virus-induced gene silencing in tomato, the signaling cascade downstream of Ve1 is shown to require both EDS1 and NDR1. In addition, NRC1, ACIF, MEK2, and SERK3/BAK1 also act as positive regulators of Ve1 in tomato. In conclusion, Ve1-mediated resistance signaling only partially overlaps with signaling mediated by Cf proteins, type members of the receptor-like protein class of resistance proteins.