DETECTION OF SINGLET OXYGEN PRODUCTION BY ESR
DETECTION OF SINGLET OXYGEN PRODUCTION BY ESR
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DOI：
10.1038/279450a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
WOLD, E
中科院分区：
文献类型：
--
作者：
MOAN, J;WOLD, E
单线态氧分子是一种非常强的氧化剂。它的作用在多种化学和生物过程中很重要1 -4，例如脂质、蛋白质和核酸的染料敏化光氧化4，病毒5和细胞4的光动力灭活，癌症的光疗6，7，致癌作用8，嗜酸性细胞的溶血9，人类皮肤的敏化4和食物的降解4。用于检测单线态氧的方法是非特异性的，灵敏度低或费力。1，3-二苯基异苯并呋喃的光氧化似乎是最广泛使用的1 O2诊断试验。然而，在没有额外的对照实验的情况下，该试验不能证明1 O2（参考文献4）和1，3-二苯基异苯并呋喃的中间体在水溶液中溶解度非常低并发生二聚反应。Lionet等人10提出了一种新的方法来检测1 O2，其中包括当1 O2与空间位阻胺2，2，6，6-四甲基哌啶反应时产生稳定的氮氧自由基。当用此方法检测中性水溶液中的1 O2时，我们没有发现自由基的产生。我们在这里报告我们对这个问题的调查，因为它是生物学上重要的是能够检测1 O2生产在这样的解决方案。
SINGLET molecular oxygen is a very powerful oxidant. Its action is important in a variety of chemical and biological processes1–4, for examples dye-sensitised photooxidation of lipids, proteins and nucleic acids4, photodynamic inactivation of viruses5and cells4, phototherapy of cancer6,7, carcinogenesis8, haemolysis of erythocytes9, sensitisation of the human skin4and degradation of food4. The methods used to detect singlet oxygen are unspecific, of low sensitivity or laborious. Photooxidation of 1,3-diphenylisobenzofuran seems to be the most widely used diagnostic test for1O2. However, in the absence of additional control experiments this test does not prove the intermediacy of1O2(ref. 4) and 1,3-diphenylisobenzofuran has very low solubility and dimerises in aqueous solutions. Lionet al.10have proposed a new method to detect1O2involving the generation of stable nitroxide radicals when1O2reacts with the sterically hindered amine 2,2,6,6,-tetramethylpiperidin. When using this method to detect1O2in neutral aqueous solutions, we found no radical production. We report here our investigation of this problem, as it is biologically important to be able to detect1O2production in such solutions.