Immunocytochemical localization of sGnRH and cGnRH‐II in the brain of goldfish, Carassius auratus

Immunocytochemical localization of sGnRH and cGnRH‐II in the brain of goldfish, Carassius auratus
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DOI:
10.1002/cne.903560105
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发表时间:
1995-05
影响因子:
2.5
通讯作者:
Myung-Hee Kim;Y. Oka;M. Amano;Makito Kobayashi;K. Okuzawa;Y. Hasegawa;S. Kawashima;Yuzuru Suzuki;K. Aida
Myung-Hee Kim;Y. Oka;M. Amano;Makito Kobayashi;K. Okuzawa;Y. Hasegawa;S. Kawashima;Yuzuru Suzuki;K. Aida
中科院分区:
医学3区
文献类型:
--
作者:
Myung-Hee Kim;Y. Oka;M. Amano;Makito Kobayashi;K. Okuzawa;Y. Hasegawa;S. Kawashima;Yuzuru Suzuki;K. Aida

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使用各自的抗血清检查金鱼脑中鲑鱼促性腺激素释放激素(sGnRH)和鸡GnRH-II(cGnRH-II)神经元的免疫细胞化学分布。鲑鱼GnRH免疫反应(ir)细胞体位于嗅神经和嗅球(终末神经节)之间的区域、端脑腹侧、视前区和下丘脑。在与sGnRH相同的区域中观察到鸡GnRH-II-ir细胞体,但细胞体数量少于sGnRH。此外,在未发现sGnRH-ir胞体的中脑被盖中也观察到鸡GnRH-II-ir胞体,sGnRH-ir和cGnRH-II-ir纤维不仅分布于下丘脑和垂体,而且还分布于从嗅球到脊髓的各个脑区。
The immunocytochemical distribution of salmon gonadotropin‐releasing hormone (sGnRH) and chicken GnRH‐II (cGnRH‐II) neurons in the brain of goldfish was examined using respective antisera. Salmon GnRH‐immunoreactive (ir) cell bodies were localized in the area between the olfactory nerve and the olfactory bulb (the terminal nerve ganglion), the ventral telencephalon, the preoptic area, and the hypothalamus. Chicken GnRH‐II‐ir cell bodies were observed in the same areas as were those of sGnRH, although the number of cell bodies were fewer than those of sGnRH. In addition, chicken GnRH‐II‐ir cell bodies were also observed in the midbrain tegmentum where no sGnRH‐ir cell bodies were found. Both sGnRH‐ir and cGnRH‐II‐ir fibers were distributed not only in the hypothalamus and the pituitary gland but also in various brain areas from the olfactory bulb to the spinal cord.