Systematic Study of Inherent Antibacterial Properties of Magnesium-based Biomaterials
Systematic Study of Inherent Antibacterial Properties of Magnesium-based Biomaterials
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DOI：
10.1021/acsami.6b02241
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发表时间：
2016-04-20
影响因子：
9.5
通讯作者：
Chu, Paul K.
中科院分区：
文献类型：
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作者：
Feng, Hongqing;Wang, Guomin;Chu, Paul K.
镁基材料由于其生物降解性、机械性能和固有的抗菌性能而优选用于临时骨科植入物。然而，细菌杀灭的基本机制和各种因素的作用尚不清楚。在这项研究中，我们进行了系统的研究，两种常见的镁基材料的抗菌性能使用的生物膜形成细菌。在0.1 mL LB培养基中，两种材料在24 h内实现了初始3 × 10（4）细菌的完全消灭，而在对照中，它们增殖至10（10）。在溶液中比在表面上更有效地杀死细菌，并且细菌杀死效率更多地取决于镁离子和氢氧根离子的浓度而不是腐蚀速率。使用配方溶液再现了杀灭过程，并揭示了杀灭源于碱度和镁离子的协同效应，而不是其中之一或Mg（OH）（2）沉淀。活性氧物质（ROS）在杀灭过程中从细菌中检测到，但不太可能直接由氧化还原反应产生，因为它们在反应开始后至少3小时才被检测到。平均细胞大小在杀灭过程中增加，表明细菌难以正常分裂，这也有助于减少细菌种群。
Magnesium-based materials are preferred in temporary orthopedic implants because of their biodegradability, mechanical properties, and intrinsic antibacterial properties. However, the fundamental mechanism of bacteria killing and roles of various factors are not clearly understood. In this study, we performed a systematic study of the antibacterial properties of two common Mg-based materials using a biofilm forming bacterium. Complete annihilation of the initial 3 X 10(4) bacteria is achieved with both materials in 0.1 mL LB medium in 24 h, whereas in the control, they proliferate to 10(10). The bacteria are killed more effectively in the solution than on the surface, and the bacteria killing efficiency depends more on the concentrations of the magnesium ions and hydroxyl ions than the corrosion rate. The killing process is reproduced using formula solutions, and killing is revealed to stem from the synergetic effects of alkalinity and magnesium ions instead of either one of them or Mg(OH)(2) precipitate. Reactive oxygen species (ROS) are detected from the bacteria during the killing process but are not likely produced by the redox reaction directly, because they are detected at least 3 h after the reaction has commenced. The average cell size increases during the killing process, suggesting that the bacteria have difficulty with normal division which also contributes to the reduced bacteria population.