Study of the pH influence on the optical properties of dissolved organic matter using fluorescence excitation-emission matrix and parallel factor analysis
Study of the pH influence on the optical properties of dissolved organic matter using fluorescence excitation-emission matrix and parallel factor analysis
复制标题
DOI：
10.1016/j.jlumin.2013.02.052
复制
发表时间：
2013-10-01
影响因子：
3.6
通讯作者：
Wang, Dongsheng
中科院分区：
文献类型：
--
作者：
Yan, Mingquan;Fu, Qiangwei;Wang, Dongsheng
采用荧光激发-发射矩阵（EEM）结合平行因子分析（PARAFAC）方法研究了溶解性有机物（DOM）的光学性质。研究的DOM样品包括Suwannee河富里酸（SRFA）和北欧水库天然有机物（NRNOM）。的DOM的光学性质确定从响应于不同的电离状态的DOM荧光团的各个组的行为。的PARAFAC检查显示，有6个独立的EEM组件中的SRFA表现不同的pH值对它们的荧光强度的影响。主要EEM峰值位置（λ（ex）/λ（em）），（230，330）nm/420 nm（组分1），（250，350）nm/450 nm（组分2），（240，340）nm/460 nm（组分3）、（220，280）nm/380 nm（组分4）、（270，400）nm/490 nm（组分5）和（280，340）nm/540 nm（组分6）。然而，在NRNOM中仅观察到四种EEM组分，其与SRFA中的组分1、2、3和4相似。组分1、2、3和6属于传统的陆生类腐殖酸发色团，但由不同组分的类羧酸和类酚发色团组成;组分4可归类为主要由羧基样发色团组成的蛋白质样荧光团，组分5可归类为主要由酚类荧光团组成的微生物类腐殖酸组分。结果表明，PARAFAC分析是一种很有前途的方法来表征DOM中的精细功能。(C)2013 Elsevier B. V.保留所有权利。
This study investigated the optical properties of dissolved organic matter (DOM) using the fluorescence excitation-emission matrix (EEM) method combined with parallel factor (PARAFAC) analysis. The examined DOM samples included Suwannee River fulvic acid (SRFA) and Nordic Reservoir natural organic matter (NRNOM). The optical properties of the DOM were determined from the behavior of individual groups of DOM fluorophores responding to different ionization states. The PARAFAC examination revealed that there were six independent EEM components in the SRFA that behaved differently in terms of pH influence on their fluorescence intensity. The dominant EEM peak locations (lambda(ex)/lambda(em)) of these components were (230, 330) nm/420 nm (Component 1), (250, 350) nm/450 nm (Component 2), (240, 340) nm/460 nm (Component 3), (220, 280) nm/380 nm (Component 4), (270, 400) nm/490 nm (Component 5), and (280, 340) nm/540 nm (Component 6). However, only four EEM components were observed in the NRNOM, which are similar to Components 1, 2, 3 and 4 in the SRFA. It can be speculated that Components 1, 2, 3 and 6 can be categorized as traditional terrestrial humic-like chromophores, while they are composed of different fractions of carboxylic-like and phenolic-like chromophores; Component 4 can be categorized as protein-like fluorophores primarily composed of carboxylic-like chromophores, and Component 5 can be categorized as a microbial humic-like component that is overwhelmingly composed of phenolic-like fluorophores. The results demonstrated that PARAFAC analysis is a promising approach for characterizing the fine functions in DOM. (C) 2013 Elsevier B.V. All rights reserved.