One-step fabrication of three-dimensional porous calcium carbonate-chitosan composite film as the immobilization matrix of acetylcholinesterase and its biosensing on pesticide
One-step fabrication of three-dimensional porous calcium carbonate-chitosan composite film as the immobilization matrix of acetylcholinesterase and its biosensing on pesticide
复制标题
一步法制备三维多孔碳酸钙-壳聚糖复合膜作为乙酰胆碱酯酶固定化基质及其农药生物传感
DOI：
10.1016/j.elecom.2009.08.005
复制
发表时间：
2009-10-01
影响因子：
5.4
通讯作者：
Zhang, Lizhi
中科院分区：
文献类型：
--
作者：
Gong, Jingming;Liu, Ting;Zhang, Lizhi
本文报道了一步共电沉积法制备的新型纳米碳酸钙-壳聚糖（nanoCaCO（3）-chi）复合膜。所制备的纳米CaCO（3）基质具有三维多孔网状结构，为生物降解酶提供了良好的生物相容性微环境。以该复合膜为酶固定化基质，制备了一种高灵敏度、高稳定性的乙酰胆碱酯酶（AChE）传感器，并将其应用于有机磷农药（OPs）模型化合物--甲基对硫磷的测定。甲基对硫磷的浓度在0.005 ~ 0.2 ~ 0.75 ~ 3.75 μ g·mL ~（-1）范围内，其抑制作用与浓度成正比。检出限低至1 ng mL（-1）（S/N = 3）。所设计的生物传感器具有良好的重现性和可接受的稳定性。(C)2009 Elsevier B. V.保留所有权利。
This work reports on a novel nanosized calcium carbonate-chitosan (nanoCaCO(3)-chi) composite film fabricated by a one-step co-electrodeposition method. The generated nanoCaCO(3)-based matrix possessed a three-dimensional (M) porous, network-like structure, providing a favorable and biocompatible microenvironment to immobilize enzyme. By using such a composite film as enzyme immobilization matrix, a highly sensitive and stable acetylcholinesterase (AChE) sensor was achieved for determination of methyl parathion as a model of organophosphate pesticides (OPs) compounds. The inhibition of methyl parathion was proportional to its concentration ranging from 0.005-0.2 to 0.75-3.75 mu g mL(-1). The detection limit was found to be as low as 1 ng mL(-1) (S/N = 3). The designed biosensor exhibited good reproducibility and acceptable stability. (C) 2009 Elsevier B.V. All rights reserved.