Cell subpopulations dispersed from solid tumours and separated by centrifugal elutriation.
Cell subpopulations dispersed from solid tumours and separated by centrifugal elutriation.
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DOI：
10.1038/bjc.1981.154
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发表时间：
1981-07
影响因子：
8.8
通讯作者：
Wheeler, K T
中科院分区：
文献类型：
--
作者：
Siemann, D W;Lord, E M;Keng, P C;Wheeler, K T
在放射生物学研究中常用的3种体内-体外肿瘤模型中评估了非肿瘤宿主细胞浸润的程度：EMT 6/Ro乳腺癌、9 L/Ro肿瘤和KHT肉瘤。虽然前两种肿瘤模型在皮下生长时显示出中度至高度免疫原性，KHT肉瘤显然是无免疫原性。使用酶解离实体瘤的单细胞悬液的差异染色，发现所有3种肿瘤类型均含有大比例（30-60%）的非肿瘤宿主细胞。在细胞悬液中发现的实际宿主细胞成分在研究的3种肿瘤的类型和百分比上都不同。从实体瘤制备的细胞悬浮液中的这些宿主细胞和肿瘤细胞可以通过离心淘洗容易地分离。分离后，评估肿瘤细胞的克隆形成潜力，发现其克隆形成能力高于未分离细胞悬液的克隆形成能力，其因子与宿主/肿瘤细胞比率直接相关。即使在去除宿主细胞后，肿瘤性EMT 6和9 L肿瘤细胞的克隆形成能力也低于相应的体外亚系的克隆形成能力（约30%对75%）。然而，在KHT肉瘤中，去除宿主细胞成分将平板接种效率提高到约60%，这与该肿瘤的体外细胞亚系的值相似。
The degree of non-neoplastic host-cell infiltration was assessed in 3 in vivo-in vitro tumour models commonly used in radiobiological studies: EMT6/Ro mammary carcinoma, 9L/Ro tumour and KHT sarcoma. While the 2 former tumour models have been shown to be moderately to highly immunogenic when grown s.c., the KHT sarcoma is apparently non-immunogenic. Using differential staining on single-cell suspensions from enzymatically dissociated solid tumours, all 3 tumour types were found to contain large proportions (30-60%) of non-neoplastic host cells. The actual host-cell component found in the cell suspensions differed both in type and percentage for the 3 tumours studied. These host and neoplastic cells in the cell suspensions prepared from the solid tumours could be readily separated by centrifugal elutriation. After separation the clonogenic potential of the neoplastic cells was assessed, and was found to be higher than the clonogenic capacity of the unseparated cell suspension by a factor directly related to the host/neoplastic cell ratio. Even after the removal of the host cells, the clonogenic capacities of the neoplastic EMT6 and 9L tumour cells were lower than that of the corresponding in vitro sublines (approximately 30 vs 75%). However, in the KHT sarcoma the removal of the host cell component raised the plating efficiency to approximately 60%, which was similar to the value for the in vitro cell subline of this tumour.