Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy
Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy
复制标题
基于超材料增强太赫兹光谱的细胞活力和水合测定
DOI：
10.1039/c7ra09609g
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Fu, Weiling
中科院分区：
文献类型：
--
作者：
Liu, Yu;Tang, Mingjie;Fu, Weiling
作为一项快速发展的技术，太赫兹时域光谱学（THz-TDS）在生物领域的应用越来越普遍，其目标是从生物分子到组织的一系列生物材料。然而，THz-TDS在细胞水平上的研究相当有限。因此，本文开展并报道了一项以无标记方式分析肿瘤细胞的生存状态和水合状态的研究。结合特殊设计的太赫兹超材料，在一段时间内连续检测肿瘤细胞单层。此外，为了探讨超物质对肿瘤细胞可能的影响，我们检测了细胞上清液中IL-6、IL-8、GM-CSF和GROα的分泌情况。结果表明，该技术可以通过监测细胞外水分来表征肿瘤细胞的生存状态，实时研究肿瘤细胞内部的水化状态，在正常细胞和不同恶性程度的肿瘤细胞的无标记检测中具有很大的应用潜力。
As a fast-growing technology, terahertz time-domain spectroscopy (THz-TDS) is becoming increasingly pervasive in biological applications, targeting a range of biomaterials from biomolecules to tissues. However, THz-TDS studies at the cellular level are quite limited. Thus, a study to analyze the living state and hydration state of a tumor cell in a label-free manner is carried out and reported here. Combined with a specially designed THz metamaterial, a tumor cell monolayer was detected continuously over a period of time. In addition, in order to explore the possible impact of the metamaterial on tumor cells, the secretion of IL-6, IL-8, GM-CSF and GROα of cell supernatants was detected. The results demonstrated that the technology could characterize the living state by monitoring the extracellular water and investigate the hydration state inside a tumor cell in real time, showing great application potential for the label-free detection of normal cells and tumor cells of diverse malignant degree.