Immunocytochemical localization of chromogranin A and secretogranin II in female rat gonadotropes.
Immunocytochemical localization of chromogranin A and secretogranin II in female rat gonadotropes.
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雌性大鼠促性腺激素中嗜铬粒蛋白 A 和分泌粒蛋白 II 的免疫细胞化学定位。
DOI：
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发表时间：
1998
影响因子：
--
通讯作者：
Y. Uchiyama
中科院分区：
文献类型：
--
作者：
T. Watanabe;T. Azuma;T. Banno;T. Jeziorowski;Y. Ohsawa;S. Waguri;D. Grube;Y. Uchiyama
已知垂体促性腺激素的超微结构显示出显著的性别相关差异：典型的雄性大鼠促性腺激素包含大颗粒和小颗粒，而典型的雌性大鼠促性腺激素似乎表现出均匀的小颗粒。我们以前的研究表明，两个代表性的颗粒，嗜铬颗粒蛋白A（CgA）和分泌颗粒蛋白II（SgII），分别定位于每种类型的颗粒在雄性大鼠促性腺激素。为了阐明颗粒蛋白和雌性大鼠促性腺激素分泌颗粒的特征之间是否存在一定的关系，我们研究了细胞中CgA和SgII的表达水平和免疫细胞化学定位。北方印迹和免疫印迹分析表明，CgA和SgII都是在雌性垂体中合成和储存的，尽管雌性垂体中CgA的量远低于雄性垂体。免疫细胞化学观察澄清，促性腺激素在女性垂体具有中间分泌颗粒含有CGA和SgII，除了单独的CGA阳性和SgII阳性的。然而，分泌颗粒中含有CGA的女性促性腺激素细胞的尺寸小得多，出现频率低于男性细胞，而没有性别差异被认为是在SgII阳性颗粒。此外，CgA阳性分泌颗粒的大小和外观不同，这取决于发情周期的阶段。这些结果表明，含有CgA的分泌颗粒的大小和外观与垂体中CgA的表达和储存水平密切相关。
Ultrastructures of pituitary gonadotropes are known to show a prominent sex-related difference: typical male rat gonadotropes contain both large- and small-sized granules, whereas typical female rat gonadotropes appear to exhibit uniformly small-sized granules. Our preceding studies have demonstrated that two representative granins, chromogranin A (CgA) and secretogranin II (SgII), are separately localized to each type of granule in male rat gonadotropes. To clarify whether or not there is a certain relationship between granin proteins and characteristic features of secretory granules in female rat gonadotropes, we examined the expression levels and immunocytochemical localizations of CgA and SgII in the cells. Northern blot and immunoblot analyses demonstrated that both CgA and SgII were synthesized and stored in the female pituitary, although the amount of CgA was much lower in the female than that in the male pituitary. Immunocytochemical observations clarified that gonadotropes in the female pituitary possessed intermediate secretory granules containing both CgA and SgII, in addition to solely CgA-positive and SgII-positive ones. However, secretory granules containing CgA in the female gonadotropes were much smaller in size and appeared less frequently than those in the male cells, whereas no sexual difference was discerned in SgII-positive granules. Moreover, the size and appearance of CgA-positive secretory granules varied depending on stages of the estrous cycle. These findings suggest that the size and appearance of secretory granules containing CgA are closely associated with the expression and storage levels of CgA in the pituitary.
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垂体 GH4C1 细胞平行分泌催乳素和促分泌素-II（调节途径的标记物）。
影响因子：
4.8
作者：
Hinkle,PM;Scammell,JG;Shanshala2nd,ED
通讯作者：
Shanshala2nd,ED
支持动情周期期间黄体生成素和卵泡刺激素非平行分泌的细胞学因素。
影响因子：
4.8
作者：
Childs,GV;Unabia,G;Tibolt,R;Lloyd,JM
通讯作者：
Lloyd,JM
重组抑制素对大鼠黄体生成素和卵泡刺激素分泌的影响。
影响因子：
4.8
作者：
Rivier,C;Schwall,R;Mason,A;Burton,L;Vaughan,J;Vale,W
通讯作者：
Vale,W
催乳素颗粒发生与 GH4C1 细胞高尔基体中分泌颗粒蛋白表达和聚集的增加有关。
影响因子：
4.8
作者：
Thompson,ME;Zimmer,WE;Haynes,AL;Valentine,DL;Forss-Petter,S;Scammell,JG
通讯作者：
Scammell,JG
通过离心淘析分离出的单个促性腺激素的黄体生成激素和卵泡释放激素的差异储存和释放。
影响因子：
4.8
作者：
Lloyd,JM;Childs,GV
通讯作者：
Childs,GV