A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay
A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay
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DOI：
10.1039/d0ay01459a
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发表时间：
2020-10-28
影响因子：
3.1
通讯作者：
Ukita, Yoshiaki
中科院分区：
文献类型：
--
作者：
Abe, Takaaki;Okamoto, Shunya;Ukita, Yoshiaki
本文报道了一种便携式免疫分析系统，该系统由一个小型的离心式微流控装置驱动器(便当盒)和一个由聚丙烯制成的注塑成型的离心式微流控装置组成。便当盒由一台廉价的直流电机和一台Arduino微控制器组成。它的结构简单，大小和便当盒子差不多，即150x150x100(宽x深x高)毫米(3)。由于该装置是根据我们先前提出的时钟原理设计的，因此它可以在稳定的旋转条件下自动执行酶联免疫吸附试验(ELISA)过程。在这里，我们首先使用由便当盒和由聚二甲基硅氧烷(PDMS)制成的装置组成的系统执行ELISA法，并将其与伺服控制的装置驱动器进行比较。经验证，便当盒的测试结果与伺服控制驱动程序的测试结果一致。使用便当盒的检出限为0.759 ng ml(-1)。从而论证了便当盒的可控性。接下来，我们通过多步流体控制对注射成型装置进行了评估。我们通过对设备的实时观察，确认在设计的ELISA程序中执行了准确的流量控制。最后，利用由便当盒和聚丙烯装置组成的检测系统，对小鼠的免疫球蛋白进行了检测。该系统在12分钟内进行了所有的流体控制；我们确认了该系统的特异性，其最低检出限为0.320 ng ml(-1)。
In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding. The bento box consists of a cheap DC motor and an Arduino microcontroller. It has a simple structure and is the size of a bento box, that is, 150 x 150 x 100 (W x D x H) mm(3). The developed device can automatically execute an enzyme-linked immunosorbent assay (ELISA) process under a steady rotating condition because it was designed based on the principle of CLOCK, which we previously presented. Here, we first executed an ELISA using a system consisting of the bento box and a device made of polydimethylsiloxane (PDMS) and compared it with a servo-controlled device driver. It was confirmed that the results of the bento box were consistent with those of the servo-controlled device driver. The limit of detection (LOD) using the bento box was 0.759 ng ml(-1). Therefore, the controllability of the bento box was demonstrated. Next, we evaluated the injection-molded device through multi-step fluid control. We confirmed, through real-time observation of the device, that accurate flow control in the designed ELISA procedure was executed. Lastly, ELISA was employed for the measurements of mouse IgG using the system consisting of the bento box and the polypropylene device. The system performed all fluidic controls within 12 min; we confirmed the specificity of the system, and the LOD was 0.320 ng ml(-1).