Establishment of a persistent Escherichia coli reservoir during the acute phase of a bladder infection
Establishment of a persistent Escherichia coli reservoir during the acute phase of a bladder infection
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DOI：
10.1128/iai.69.7.4572-4579.2001
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发表时间：
2001-07-01
影响因子：
3.1
通讯作者：
Hultgren, SJ
中科院分区：
文献类型：
--
作者：
Mulvey, MA;Schilling, JD;Hultgren, SJ
绝大多数尿路感染是由编码丝状粘附细胞器的尿路致病性大肠杆菌菌株引起的，这种细胞器称为I型药丸。这些结构介导细菌附着和侵入膀胱上皮细胞。然而，1型菌毛介导的细菌入侵导致尿路感染的发病机制尚不清楚。在这里，我们表明，1型piliated尿路病原体可以侵入浅表上皮细胞，内衬膀胱腔表面，随后复制，形成大量的细胞内大肠杆菌称为细菌工厂的焦点。作为对感染的反应，浅表膀胱细胞脱落并随尿液排出。为了避免脱落清除，细胞内的尿路病原体可以重新出现，并最终建立一个持久的，静止的细菌水库内膀胱粘膜，可能作为一个来源，复发性急性感染。这些观察结果表明，尿路感染比通常认为的更慢性和侵袭性。
The vast majority of urinary tract infections are caused by strains of uropathogenic Escherichia coli that encode filamentous adhesive organelles called type I pill. These structures mediate both bacterial attachment to and invasion of bladder epithelial cells. However, the mechanism by which type 1 pilus-mediated bacterial invasion contributes to the pathogenesis of a urinary tract infection is unknown. Here we show that type 1-piliated uropathogens can invade the superficial epithelial cells that line the lumenal surface of the bladder and subsequently replicate, forming massive foci of intracellular E, coli termed bacterial factories. In response to infection, superficial bladder cells exfoliate and are removed with the how of urine. To avoid clearance by exfoliation, intracellular uropathogens can reemerge and eventually establish a persistent, quiescent bacterial reservoir within the bladder mucosa that may serve as a source for recurrent acute infections. These observations suggest that urinary tract infections are more chronic and invasive than generally assumed.