A high-performance liquid chromatography method for the assay of cytidine monophosphate-sialic acid synthetase.
A high-performance liquid chromatography method for the assay of cytidine monophosphate-sialic acid synthetase.
复制标题
高效液相色谱法测定胞苷单磷酸唾液酸合成酶。
DOI：
10.1016/0003-2697(83)90147-1
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Korytnyk,W
中科院分区：
文献类型：
--
作者：
Petrie3rd,CR;Korytnyk,W
建立了用离子对色谱和梯度洗脱法测定胞苷单磷酸-唾液酸合成酶（EC 2.7.7.43）的高效液相色谱法。该程序允许测定酶的替代底物和抑制剂，并且不受比色法的限制。新合成的n -乙酰-9-脱氧-9-氟-d-神经氨酸是该酶的良好底物，其Kmof为6.35 mm，而n -乙酰神经氨酸的Kmof为1.84 mm。
An HPLC method has been developed for the assay of cytidine monophosphate-sialic acid synthetase (EC 2.7.7.43) using ion-pair chromatography and gradient elution. This procedure permits the assay of alternative substrates and inhibitors of the enzyme and is not subject to the limitation of the colorimetric method. The newly synthesized N-acetyl-9-deoxy-9-fluoro-d-neuraminic acid was found to be a good substrate of the enzyme with a Kmof 6.35 mm as compared to 1.84 mm for N-acetylneuraminic acid.