Expression of Osteocalcin During Surgically Assisted Rapid Orthodontic Tooth Movement in Beagle Dogs
Expression of Osteocalcin During Surgically Assisted Rapid Orthodontic Tooth Movement in Beagle Dogs
复制标题
比格犬手术辅助快速正畸牙齿移动过程中骨钙素的表达
DOI：
10.1016/j.joms.2008.06.087
复制
发表时间：
2008-12-01
影响因子：
1.9
通讯作者：
Bai, Ding
中科院分区：
文献类型：
--
作者：
Han, Xiang-Long;Meng, Yao;Bai, Ding
目的：探讨比格犬在牙槽手术辅助下快速正畸牙齿移动过程中骨钙素的表达。材料与方法：选取8只雄性beagle犬，用正畸NiTi螺旋弹簧以100 gm的力拔除双侧下颌第二前磨牙，将第一前磨牙与第三前磨牙进行远端分离。将左右下颌侧随机分为实验侧和假侧，仅在实验侧进行牙槽手术，以减少拔牙槽内近侧骨阻力。前4周每周测量1次牙齿移动距离。分别于1、2、4、8周后处死2只小猎犬，通过免疫组织化学检测骨钙素的表达，并用计算机辅助图像分析进行定量分析。结果：实验侧第一前磨牙在41周内的平均总移动量（1.868 +/- 0.022 mm）约为假牙侧（1.008 +/- 0.057 mm）的两倍。在平均总锚固损失方面，两组间差异无统计学意义（P < 0.05）。骨钙素在4个时间段的动态浓度变化（P < 0.05）。实验侧骨钙素染色强度高于相应的假侧，受压部位骨钙素染色强度高于相应的张力部位（P < 0.05）。结论：骨钙素的表达表明，牙槽骨手术是一种有效、安全的促进正畸牙移动、促进骨重塑的方法。(C) 2008年美国口腔颌面外科医师协会
Purpose: To investigate the expression of osteocalcin during rapid orthodontic tooth movement aided by alveolar surgery in beagle dogs.Materials and Methods: Eight male beagles were used, and bilateral mandibular second premolars were extracted for distalization of the first premolars against the third premolars by orthodontic NiTi coil spring with a force of 100 gm. Right and left mandibular sides were randomly assigned to experimental and sham sides, and alveolar surgery was performed only on the experimental side to reduce the osteal resistance on the mesial side of the extraction socket. Distance of tooth movement was measured once a week during the first 4 weeks. Two beagles were sacrificed after 1, 2, 4, and 8 weeks of orthodontic force application, and expression of osteocalcin was demonstrated by immunohistochemistry and quantified with computer-aided image analysis.Results: The average total movement of the first premolars in 41 weeks on the experimental side (1.868 +/- 0.022 mm) was approximately double that on the sham side (1.008 +/- 0.057 mm). As regards the average total anchorage loss, no significant difference (P > .05) was revealed. Dynamic osteocalcin concentrations presented at 4 time periods (P < .05). The staining intensity of osteocalcin on the experimental sides was higher than the corresponding sham sides, and that on the compression sites higher than the corresponding tension sites (P < .05).Conclusions: The results suggested that alveolar surgery might serve as an effective and safe way to accelerate orthodontic tooth movement, and promote the rate of bone remodeling as revealed by the expression of osteocalcin. (C) 2008 American Association, of Oral and Maxillofacial Surgeons