Effects of coumestrol administration to pregnant and lactating mice on intestinal alkaline phosphatase activit
Effects of coumestrol administration to pregnant and lactating mice on intestinal alkaline phosphatase activit
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香雌酚对妊娠和哺乳期小鼠肠道碱性磷酸酶活性的影响
DOI：
10.1002/ptr.3317
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发表时间：
2011
影响因子：
7.2
通讯作者：
Kume S.
中科院分区：
文献类型：
--
作者：
Kirihata Y;Horiguchi Y;Ueda M;Sugimoto M;Ikeda S;Kume S.
本研究旨在阐明香豆雌酚对妊娠期和哺乳期小鼠钙代谢的影响。交配后6.5 - 16.5天（dpc），妊娠小鼠接受200 µg/kg体重/天的香豆雌酚给药。在出生后17.5天或分娩后10天（dap）采集十二指肠、空肠和血液样品。香豆雌酚给药可降低分娩前小鼠十二指肠和空肠中碱性磷酸酶（ALP）活性以及IAP和雌激素反应基因c-fos和血管内皮生长因子（VEGF）的mRNA表达。在哺乳期小鼠中，ALP活性和IAP的mRNA表达没有改变，尽管香豆雌酚给药降低了十二指肠c ‐fosin和空肠VEGF的mRNA表达。香豆雌酚不影响血清钙和维生素D受体蛋白在十二指肠和空肠的表达。因此，妊娠期给予香豆雌酚可能通过ERα降低分娩前小鼠肠道IAP的mRNA表达和ALP活性，但在分娩后10天，香豆雌酚对肠道ALP活性几乎没有影响。版权所有© 2010约翰威利父子有限公司.
The present study was conducted to clarify the effects of coumestrol administration on Ca metabolism during pregnancy and in lactating mice. From 6.5 to 16.5 days post coitus (dpc), pregnant mice were administered coumestrol at 200 µg/kg body weight/day. The duodenum, jejunum and blood samples were obtained at 17.5 dpc or 10 days after parturition (dap). Coumestrol administration decreased alkaline phosphatase (ALP) activity and mRNA expression ofIAPand estrogen responsive genes,c‐fosand vascular endothelial growth factor (VEGF), in the duodenum and jejunum of pre‐delivery mice. In lactating mice, the ALP activity and mRNA expression ofIAPwere not changed, although coumestrol administration decreased mRNA expression ofc‐fosin the duodeum andVEGFin the jejunum. Coumestrol did not affect serum Ca and the expression of vitamin D receptor protein in the duodenum and jejunum. Thus, coumestrol administration during pregnancy may decrease the mRNA expression ofIAPand the ALP activity in the intestine of the pre‐delivery mice through ERα, but coumestrol had little effect on intestinal ALP activity at 10 days after parturition. Copyright © 2010 John Wiley & Sons, Ltd.