Influence of surface charges on the rupture of black lipid membranes
Influence of surface charges on the rupture of black lipid membranes
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DOI：
10.1103/physreve.58.4883
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发表时间：
1998-10-01
影响因子：
2.4
通讯作者：
Winterhalter, M
中科院分区：
文献类型：
--
作者：
Diederich, A;Bahr, G;Winterhalter, M
我们研究了表面电荷对黑脂膜（BLM）破裂的影响。破裂是由跨膜的短电压脉冲引起的。诱导击穿所需的平均电压给出了关于缺陷形成的能垒的信息。在破裂过程中跨膜电压的快速衰减允许人们评估缺陷加宽的动力学。对于由具有磷脂酰丝氨酸头基的脂质制成的BLM和由具有磷脂酰胆碱头基的脂质制成的BLM，击穿电压大致相同。此外，击穿电压与包围BLM的水性介质的离子强度无关。这表明BLM的稳定性不是由头基之间的相互静电排斥决定的。然而，击穿电压取决于脂质的疏水链的类型。棕榈酰-油酰（PO）膜需要比二植烷酰（DPh）膜小100 mV的击穿电压。令人惊讶的是，断裂动力学取决于烃链。它被发现是四倍快DPh比PO膜，和独立的头基的类型。[S1063-651X（98）15210-8]。
We investigate the influence of surface charges on the rupture of black lipid membranes (BLM). Rupture is induced by short electric voltage pulses across the membrane. The average voltage necessary to induce breakdown gives information on the energy barrier of defect formation. The rapid transmembrane voltage decay during rupture allows one to evaluate the kinetics of defect widening. The breakdown voltage is about the same for BLM made of lipids with phosphatidylserine headgroups and for BLM made of lipids with phosphatidylcholine headgroups. Moreover, the breakdown voltage is independent of the ionic strength of the aqueous medium surrounding the BLM. This indicates that the stability of the BLM is not dominated by mutual electrostatic repulsion of the headgroups. However, the breakdown voltage depends on the type of the hydrophobic chains of the lipids. Palmitoyl-oleoyl (PO) membranes require similar to 100-mV smaller breakdown voltages compared to diphytanoyl (DPh) membranes. Surprisingly, the rupture kinetics depends on the hydrocarbon chains. It was found to be four times faster for DPh than for PO membranes, and independent of the type of headgroup. [S1063-651X(98)15210-8].