Detection and quantification of the age-related sjTREC decline in human peripheral blood
Detection and quantification of the age-related sjTREC decline in human peripheral blood
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人外周血中与年龄相关的 sjTREC 下降的检测和定量
DOI：
10.1007/s00414-010-0528-3
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发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Wu, Xinyao
中科院分区：
文献类型：
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作者：
Ou, Xueling;Zhao, Hu;Wu, Xinyao
人外周血中信号联合T细胞受体重排切除环（sjTRECs）的下降与年龄有关，可作为个体年龄测定的潜在指标。然而，很少有人知道sjTREC水平和年龄之间的定量关系。本研究旨在探讨sjTREC在中国不同年龄人群外周血白细胞（PBLs）中的表达水平，探讨其是否可作为法医学生物学年龄推断的指标。通过实时定量PCR分析测量来自已知年龄的个体（n= 248）的外周血样品中的sjTREC水平。定量结果显示，sjTREC在人外周血淋巴细胞中呈年龄依赖性下降（r=-0.8177，P < 0.01）。基于外周sjTREC下降的年龄估计公式为Y = −24.921x− 39.932 ± 10.47（Yage，岁;Xlog sjTREC/TBP; 10.47：标准误）。此外，在sjTREC水平方面，男性和女性之间没有差异。这些结果表明，sjTREC在PBL的评估可能是一个有价值的额外的工具，在年龄确定，特别是在案件中，传统的形态学信息是缺乏或效率低下的法医实践。
The decline of signal joint T-cell receptor rearrangement excision circles (sjTRECs) in human peripheral blood has been demonstrated to be age-related, which can be a potential marker for individual age determination. However, little is known about the quantitative relationship between the levels of sjTREC and age. The aim of the present study was to investigate the levels of sjTREC in peripheral blood leukocytes (PBLs) among different age groups in Chinese population, so as to clarify whether it could serve as a suitable marker for biological age estimation in forensic practice. sjTREC levels were measured by real-time quantitative PCR analysis in peripheral blood samples from individuals of known age (n= 248). The quantification results showed that sjTREC declined in human PBLs in an age-dependent manner (r= −0.8177,P< 0.01). The formula for age estimation based on peripheral sjTREC decline wasY= −24.921x− 39.932 ± 10.47 (Yage, year;Xlog sjTREC/TBP; 10.47: standard error). Furthermore, there was no difference between males and females with regard to sjTREC levels. These results suggest that assessment of sjTREC in PBLs might be a valuable additional tool in age determination, especially in cases where traditional morphologic information is absent or inefficient in forensic practice.