Ultrastructure of sarcoma 180 cells after ultrasound irradiation in the presence of sparfloxacin.
Ultrastructure of sarcoma 180 cells after ultrasound irradiation in the presence of sparfloxacin.
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发表时间：
2004-05
影响因子：
2
通讯作者：
Deqing Huang;K. Okada;Chiyo Komori;E. Itoi;K. Kawamura;Toshio Suzuki
中科院分区：
文献类型：
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作者：
Deqing Huang;K. Okada;Chiyo Komori;E. Itoi;K. Kawamura;Toshio Suzuki
背景迄今为止，声动力学协同抗肿瘤作用已得到充分描述，但该领域仍缺乏详细的超微结构研究。材料和方法 将肉瘤 180 细胞悬液在司帕沙星 (SPFX)（新型喹诺酮抗生素之一）存在下以 2 W (0.64 W/cm2) 暴露于超声波 (US) 30 秒。通过扫描和透射电镜观察细胞存活率和细胞形态来评价抗肿瘤效果。结果 US照射后，添加SPFX组肿瘤细胞的存活率为30.9%，显着低于对照组的78.7%（p = 0.0002）。对照组中的大多数细胞是球形的，但添加 SPFX 的组中的细胞是非球形的。细胞膜经常破裂，观察到许多不同大小的孔。有些细胞完全瓦解了。结论 US和SPFX协同抗肿瘤活性最典型的变化是在细胞膜上。
BACKGROUND Sonodynamic synergistic antitumor effects have been well described to date, but detailed ultrastructural studies in this field are still lacking. MATERIALS AND METHODS A suspension of sarcoma 180 cells was exposed to ultrasound (US) in the presence of sparfloxacin (SPFX), one of the new quinolone antibiotics, at 2 W (0.64 W/cm2) for 30 seconds. The antitumor effect was evaluated by the survival rate of cells and cell morphology by scanning and transmission electron microscopy. RESULTS After US irradiation, the survival rate of tumor cells in the SPFX-added group was 30.9%, significantly lower than 78.7% in the control group (p = 0.0002). Most cells in the control group were spherical, but in the SPFX-added group the cells were aspherical. Cell membranes were often ruptured and numerous pores of various sizes were observed. Some cells were totally disintegrated. CONCLUSION The most characteristic changes of the synergistic antitumor activities of US and SPFX were on the cell membrane.