24-epibrassinolide alleviate drought-induced photoinhibition in Capsicum annuum via up-regulation of AOX pathway
24-epibrassinolide alleviate drought-induced photoinhibition in Capsicum annuum via up-regulation of AOX pathway
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24-表油菜素内酯通过上调 AOX 通路减轻辣椒干旱诱导的光抑制
DOI：
10.1016/j.scienta.2018.09.016
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发表时间：
2019-01
影响因子：
4.3
通讯作者：
Xi Rei
中科院分区：
文献类型：
--
作者：
Hu Wen-Hai;Yan Xiao-Hong;He Yan;Xi Rei
本研究旨在探讨BRs是否能通过上调替代氧化酶（AOX）途径改善光合电子传递，从而缓解干旱诱导的辣椒叶片光抑制。本研究考察了24-表油菜素内酯（EBR, 0.1 μM）和水杨酸羟肟酸（SHAM, 1 mM）对AOX途径容量、matale/oxaloacetate （OAA）穿梭、在15%聚乙二醇（PEG）处理的干旱条件下，辣椒（Capsicum annuumL.）叶片的叶绿素荧光。干旱处理4 d后，叶片相对含水量（LRWC）由92.0%降至47.0%。干旱诱导AOX通路上调，nadp -苹果酸脱氢酶（MDH）和nadp -MDH活性升高。抑制AOX途径降低了干旱条件下叶片NADP- mdh和NADP- mdh活性，增强了叶片NADPH和NADPH/NADP+比值的升高。同时，AOX途径的抑制加剧了干旱条件下叶片PSII最大量子效率（Fv/Fm）、PSII量子效率（ФPSII）、光化学猝灭系数（qP）和光合电子传递率（ETR）的降低。上述结果表明，上调AOX通路可激活苹果酸/OAA穿梭，耗散叶绿体中多余的还原当量，从而优化PSII效率和光合电子传递。在水分充足和干旱条件下，EBR处理上调了叶片中AOX通路的活性。EBR处理也显著缓解了干旱条件下SHAM处理和未SHAM处理叶片Fv/Fm、ФPSII、qP和ETR的下降，但SHAM处理的改善程度更有效。此外，在干旱条件下，EBR显著提高了SHAM处理和未SHAM处理叶片的NADP- mdh和NADP- mdh活性，并显著降低了NADPH/NADP+比值。这些结果表明，ebr诱导的抗光抑制作用部分与AOX途径的上调有关，AOX途径通过耗散过量的还原等量物。
The aim of this study was to explore whether application of BRs could alleviate drought-induced photoinhibition in pepper leaves by improving the photosynthetic electron transport via up-regulation of alternative oxidase (AOX) pathway, we examined the effects of 24-epibrassinolide (EBR, 0.1 μM) and salicylhydroxamic acid (SHAM, 1 mM) on the AOX pathway capacity, matale/oxaloacetate (OAA) shuttle, and chlorophyll fluorescence in pepper (Capsicum annuumL.) leaves under drought condition (treated by 15% polyethylene glycol, PEG). In this study, the leaf relative water content (LRWC) decreased from 92.0% to 47.0% by drought treatment for 4 d. Drought induced the up-regulation of AOX pathway and increased the activities of NADP-malate dehydrogenase (MDH) and NAD-MDH. Inhibition of AOX pathway decreased NADP-MDH and NAD-MDH activities and enhanced the increases of NADPH and NADPH/NADP+ratio in leaves under drought condition. Meanwhile, inhibition of AOX pathway aggravated the decreases of maximal quantum efficiency of PSII (Fv/Fm), quantum efficiency of PSII (ФPSII), photochemical quenching coefficient (qP), and photosynthetic electron transport rate (ETR) in leaves under drought condition. These results indicated that up-regulation of AOX pathway could activate malate/OAA shuttle and dissipate the excess reducing equivalents in chloroplast, resulting in an optimization of PSII efficiency and photosynthetic electron transport. EBR treatment up-regulated the activity of AOX pathway in leaves under well-watered and drought conditions. EBR treatment also significantly alleviated the decreases of Fv/Fm, ФPSII, qP, and ETR in leaves with/without SHAM treatment under drought condition, however, the improvement degree was more efficiently in leaves with SHAM treatment. Furthermore, EBR increased the activities of NADP-MDH and NAD-MDH, and significantly decreased NADPH/NADP+ratio in leaves with/without SHAM treatment under drought condition. These results suggested that EBR-induced protection against photoinhibition was partly related to the up-regulation of AOX pathway by dissipating excess reducing equivalents.
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影响因子：
2.3
作者：
J. Yu;Y. Matsui
通讯作者：
J. Yu;Y. Matsui
DOI：
10.1111/j.1365-3040.2011.02384.x
发表时间：
2011-11
期刊：
Plant, cell & environment
影响因子：
--
作者：
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通讯作者：
Keisuke Yoshida;C. Watanabe;I. Terashima;K. Noguchi
影响因子：
4.3
作者：
Hu, Wen-hai;Yan, Xiao-hong;Ogweno, Joshua Otieno
通讯作者：
Ogweno, Joshua Otieno
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影响因子：
4.9
作者：
Huang, Wei;Zhang, Shi-Bao;Cao, Kun-Fang
通讯作者：
Cao, Kun-Fang
影响因子：
6.4
作者：
Li-tao Zhang;Zishan Zhang;Hui-Yuan Gao;Z. Xue;Cheng Yang;Xiang-Long Meng;Q. Meng
通讯作者：
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