Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates
Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates
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DOI：
10.1016/j.cplett.2009.11.032
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发表时间：
2010-01-07
影响因子：
2.8
通讯作者：
Crain, Jason
中科院分区：
文献类型：
--
作者：
Giraud, Gerard;Schulze, Holger;Crain, Jason
在这封信中，我们演示了时间分辨荧光各向异性测量的应用，以检测链霉亲和素缀合物 (CdSe) ZnS 量子点 (QD) 的溶液态杂交。该研究是在含有 10 nM QD 和 800 nM DNA 孵育的样品上进行的。我们发现，QD-DNA 构建体的旋转相关时间在杂交后显着增加，分别为 330 ns (QD-ssDNA) 和 1.3 mu s (QD-dsDNA)，对应的直径分别为 14 nm 和 23 nm。本研究开辟了使用量子点进行杂交检测的新模式。 (C) 2009 Elsevier B.V. 保留所有权利。
In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe) ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD-DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 mu s (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots. (C) 2009 Elsevier B. V. All rights reserved.