Mapping quantitative trait loci for disease resistance to false smut of rice
Mapping quantitative trait loci for disease resistance to false smut of rice
复制标题
水稻抗黑穗病数量性状基因座定位
DOI：
10.1186/s42483-020-00059-6
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发表时间：
2020-07-30
影响因子：
3.4
通讯作者：
Sun, Wenxian
中科院分区：
文献类型：
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作者：
Han, Yanqing;Li, Deqiang;Sun, Wenxian
稻曲病是由稻曲病菌引起的一种重要的水稻新病害，在世界许多水稻产区严重影响水稻产量和品质。鉴定赋予稻曲病抗性的数量性状位点（QTL）将有助于将稻曲病抗性整合到优良品种中。本研究以抗稻曲病地方品种MR 183 -2和高感品系08 R2394为亲本，利用重组自交系定位稻曲病抗性QTL。共筛选出179个SSR多态性标记用于构建遗传连锁图谱。利用复合区间作图法，在RIL群体的第2、4、8和11染色体上检测到5个抗稻曲病QTL。在这些QTL中，位于第8染色体上的qFsr 8 - 1是解释表型变异最大的主要数量位点。该主效QTL区域的基因注释为抗病QTL的克隆提供了理论基础。与qFsr 8 - 1连锁的SSR标记可用于水稻抗稻曲病分子标记辅助育种。此外，还检测到9个抽穗期QTL。抗病性评分与抽穗期相关分析表明，稻曲病抗性与生育期长短呈负相关。
False smut of rice, an important emerging disease caused byUstilaginoidea virens, greatly reduces grain yield and deteriorates grain quality in many rice-growing regions worldwide. Identification of quantitative trait loci (QTL) conferring false smut resistance will facilitate incorporation of false smut resistance into elite cultivars. In this study, a set of recombinant inbred lines (RILs) derived from a cross of a resistant rice landrace MR183–2 and a high susceptible line 08R2394 were evaluated to map resistance QTLs for false smut. A total of 179 simple sequence repeat (SSR) polymorphic markers were identified for constructing genetic linkage maps. Using a composite interval mapping method, five false smut resistance QTLs were detected on chromosomes 2, 4, 8 and 11 in the RIL population. Among these QTLs,qFsr8–1within a small region on chromosome 8 represents a major quantitative locus explaining the greatest phenotypic variance. Gene annotation in this major QTL region provides a theoretical basis for cloning of resistance QTLs. The SSR markers genetically linked toqFsr8–1are valuable for marker-assisted breeding for false smut resistance in rice. In addition, nine QTLs for heading date were detected in this population. Correlation analysis of disease resistance score and heading date indicates that false smut resistance negatively correlates with the growth period length in rice.