CHARACTERIZATION OF AN AUTOMATED APPARATUS FOR PRECISE CONTROL OF INHALATION CHAMBER ETHANOL VAPOR AND BLOOD ETHANOL CONCENTRATIONS
CHARACTERIZATION OF AN AUTOMATED APPARATUS FOR PRECISE CONTROL OF INHALATION CHAMBER ETHANOL VAPOR AND BLOOD ETHANOL CONCENTRATIONS
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DOI：
10.1111/j.1530-0277.1986.tb05121.x
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发表时间：
1986-08-01
期刊：
影响因子：
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通讯作者：
SALEM, N
中科院分区：
文献类型：
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作者：
KARANIAN, J;YERGEY, J;SALEM, N
构建了用于小动物乙醇蒸汽暴露的具有监测和控制装置的吸入室。采用热导检测器连续测定吸入室乙醇蒸气浓度。浓度保持在非常窄的范围内（±. 1毫克/升）的设计中加入一个控制乙醇泵的反馈回路。正如预期的那样，雄性Sprague-Dawley大鼠的血液乙醇浓度（BEC）与腔室乙醇浓度呈正线性相关。当大鼠暴露于17，25或32毫克/升的室乙醇浓度为24小时，相应地低，中等，或高的平均血液乙醇水平。当一个大的人口的这一品系的大鼠（n = 121）暴露于恒定的乙醇蒸汽浓度为14天（25毫克/升）相当大的个体间的血液水平发生变化。在监测的动物中，生物等效性也随时间而存在个体差异。平均值。+-。SD BEC为189 ±。90 mg/100 ml，BEC呈类高斯分布。在BEC > 120 mg/100 ml的大鼠中，在接触酒精3.5、7或14天后观察到酒精戒断的行为特征。该装置和吸入范例使得（1）室乙醇浓度的精确控制成为可能，这显著增强了对酒精暴露期间血液乙醇水平的受试者内和受试者间波动的控制，以及（2）广泛范围的血液乙醇浓度与其生理和生化效应之间的关系的全面检查。
Inhalation chambers with a monitoring and control apparatus for ethanol vapor exposure of small animals were constructed. A thermal conductivity detector was employed for continuous measurement of inhalation chamber ethano vapor concentration. The concentration was maintained within a very narrow range (.+-. 1 mg/liter) by incorporting into the design a feedback loop which controls the ethanol pump. As expected, the blood ethanol concentration (BEC) of male Sprague-Dawley rats were positively and linearly correlated to the chamber ethanol concentration. When rats were exposed for 24 hr to a chamber ethanol concentration of 17, 25, or 32 mg/liter, corrrespondingly low, moderate, or high mean blood ethanol levels were obtained. When a large population of this strain of rats (n = 121) was exposed to a constant ethanol vapor concentration for 14 days (25 mg/liter) considerable interindividual variation in blood levels occurred. There was also individual variation over time in the BEC of animals monitored. The mean .+-. SD BEC was 189 .+-. 90 mg/100 ml for this population and a gaussian-like distribution was obtained with regard to BEC. Behaviour characteristic of alcohol withdrawal was observed in rats with BEC > 120 mg/100 ml after 3.5, 7, or 14 days of exposure. This apparatus and inhalation paradigm make possible (1) the precise control of chamber ethanol concentration which markedly enhances control over both intra- and intersubject fluctuation in blood ethanol levels during alcohol exposure and (2) the comprehensive examination of relationships between a wide range of blood ethanol concentrations and their physiological and biochemical effects.