Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
复制标题
植物血凝素：正常人白细胞培养物中有丝分裂的引发剂。
作者：
P. Nowell
摘要研究了正常人血液中白细胞在“梯度”培养中启动有丝分裂活性的可能因素。温度、pH、氧分压、二氧化碳分压、血浆和细胞浓度的变化，以及搅拌量的变化，至少在体内可能遇到的广泛范围内，仅产生有丝分裂活动的适度数量变化。粘蛋白植物提取物植物血凝素(PHA)最初被用作从全血中分离白细胞的方法，在准备培养时被发现是有丝分裂活动的特异性启动者：在它存在时，细胞发生分裂；在它不存在时，不出现有丝分裂。研究表明，PHA的促有丝分裂作用本身并不涉及有丝分裂本身，而是有丝分裂之前的阶段--循环中的单核细胞和大淋巴细胞改变到能够分裂的状态。PHA的这种促有丝分裂作用与体内有丝分裂和有丝分裂前过程的关系仍有待研究。
Summary Possible factors responsible for the initiation of mitotic activity in “gradient” cultures of leukocytes from normal human blood were investigated. Variations of temperature, pH, oxygen tension, carbon dioxide tension, plasma and cell concentrations, as well as the amount of agitation, over at least as wide a range as might be encountered in vivo , produced only moderate quantitative changes in mitotic activity. The mucoprotein plant extract, phytohemagglutinin (PHA), employed originally as a means of separating the leukocytes from whole blood in preparing the cultures, was found to be a specific initiator of mitotic activity: in its presence, cell division occurred; in its absence, no mitoses appeared. The studies suggest that the mitogenic action of PHA does not involve mitosis per se but rather the stage preceding mitosis—the alteration of circulating monocytes and large lymphocytes to a state wherein they are capable of division. The relationship of this mitogenic action of PHA to mitotic and premitotic processes in the body remains to be investigated.