HuC:Kaede, a useful tool to label neural morphologies in networks in vivo
HuC:Kaede, a useful tool to label neural morphologies in networks in vivo
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DOI：
10.1002/gene.20196
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发表时间：
2006-03-01
期刊：
影响因子：
1.5
通讯作者：
Okamoto, H
中科院分区：
文献类型：
--
作者：
Sato, T;Takahoko, M;Okamoto, H
一个简单而有效的标记神经元的过程是研究斑马鱼神经网络发育的先决条件。为了标记神经元，我们使用Kaede，这是一种具有光转换特性的荧光蛋白，在用UV或紫光照射后，其允许从绿色荧光转换为红色荧光。我们建立了一个斑马鱼稳定的转基因系，Tg（HuC：Kaede），表达Kaede在神经元的HuC启动子的控制下。该转基因系用于标记三叉神经节中的少量神经元。此外，使用注射了转基因的胚胎，我们在受精后4天标记了Rohon-Beard神经元的外周轴突，并观察了顶盖神经元的树突发育3天。这些数据表明，Kaede是选择性标记斑马鱼神经网络的有用工具。
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish.