Differential distribution of digestive enzymes in isolated epithelial cells from developing human fetal small intestine and colon.
Differential distribution of digestive enzymes in isolated epithelial cells from developing human fetal small intestine and colon.
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发育中的人胎儿小肠和结肠的分离上皮细胞中消化酶的差异分布。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Pierre Pothier
中科院分区：
文献类型：
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作者：
Daniel Ménard;Pierre Pothier
人类胎儿结肠在发育过程中具有与小肠相似的形态和生化特征。通过选择性地分离不同妊娠年龄各自的上皮，对这两个器官进行了比较研究。组织学和生化分析表明，无论其发育阶段如何，上皮都会从下面的组织中完全且选择性地去除。肠和结肠上皮细胞在蛋白质含量和分布方面表现出显着差异。研究的所有刷状缘膜酶都存在于结肠中，尽管其数量远低于相应肠道中的量。在分离的细胞中恢复了 90% 至 99% 的总二糖酶、葡糖淀粉酶和 γ-谷氨酰转肽酶活性。然而，这两种组织的上皮含量和碱性磷酸酶（ALP）活性的分布存在显着差异。 15 周时，超过 45% 的肠道 ALP 活性是非上皮来源的，并且这一百分比以头尾方式增加，在 18 周龄的结肠中达到 93% 以上。使用苯丙氨酸和左旋咪唑的抑制研究表明，这些肠道和结肠同工酶虽然各自的分布不同，但性质相似。使用上皮细胞的纯部分提供了一个理想的系统，可以在其中专门比较肠和结肠上皮的功能发育。
The human fetal colon has morphological and biochemical characteristics similar to that of the small intestine during development. A comparative study of these two organs was undertaken by selectively isolating their respective epithelium at different ages of gestation. Histological and biochemical analysis revealed a complete and selective removal of the epithelium from the underlying tissue regardless of their stage of development. Intestinal and colonic epithelial cells showed substantial differences with respect to protein content and distribution. All brush border membrane enzymes studied were present in the colon, although in much lower quantities than in the corresponding intestine. Between 90 and 99% of total disaccharidases, glucoamylase and gamma-glutamyl transpeptidase activities were recovered in the isolated cells. Epithelial content and distribution of alkaline phosphatase (ALP) activity differed markedly, however, between these two tissues. More than 45% of intestinal ALP activity was of nonepithelial origin at 15 weeks, and this percentage increased in a craniocaudal fashion to reach greater than 93% in the 18-week-old colon. Inhibition studies using phenylalanine and levamisole showed that these intestinal and colonic isoenzymes are similar in nature although they differ in their respective distribution. The use of pure fractions of epithelial cells provides an ideal system in which to compare specifically the functional development of intestinal and colonic epithelium.