TGF-β1 IS CRITICAL FOR WALLERIAN DEGENERATION AFTER RAT SCIATIC NERVE INJURY
TGF-β1 IS CRITICAL FOR WALLERIAN DEGENERATION AFTER RAT SCIATIC NERVE INJURY
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TGF-β1 对于大鼠坐骨神经损伤后的华勒氏变性至关重要
DOI：
10.1016/j.neuroscience.2014.10.051
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发表时间：
2015-01-22
期刊：
影响因子：
3.3
通讯作者：
Yao, D.
中科院分区：
文献类型：
--
作者：
Li, M.;Zhang, P.;Yao, D.
沃勒变性（WD）是损伤部位远端轴突变性的过程，随后出现强烈的再生反应。它涉及神经损伤直接诱导并由转录因子激活的变性和再生。尽管WD已被广泛研究，但转录因子调节WD的精确机制仍然难以捉摸。在本研究中，我们报道了转化生长因子-β 1 (TGF-β 1) 对大鼠坐骨神经损伤后 WD 的影响。数据显示，TGF-β1可能在损伤的大鼠坐骨神经和培养的雪旺细胞（SC）中表达。敲低 TGF-β 1 表达导致 SC 增殖和凋亡减少，细胞因子和 Smad2 上调，4。TGF-β 1 表达增强可促进 SC 增殖和凋亡，下调细胞因子和 Smad2，4。TGF-β 1 表达改变可能影响 Smad 和 AKT，但不影响 c-Jun 和细胞外调节蛋白 激酶（ERK）途径。我们的结果揭示了 TGF-β1 对 WD 的作用，并为 TGF-β1 调节神经变性和/或再生的分子机制提供了基础。 (C) 2014 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Wallerian degeneration (WD) is a process of axonal degeneration distal to the injury site followed by a robust regenerative response. It involves degeneration and regeneration which can be directly induced by nerve injury and activated by transcription factors. Although WD has been studied extensively, the precise mechanisms of transcription factors regulating WD are still elusive. In this study, we reported the effect of transforming growth factor-beta 1 (TGF-beta 1) on WD after rat sciatic nerve injury. The data showed that TGF-beta 1 may express in injured rat sciatic nerve and cultured Schwann cells (SCs). Knock down of TGF-beta 1 expressions resulted in the reduction of SC proliferation and apoptosis, up regulation of cytokines and Smad2, 4. Enhanced expression of TGF-beta 1 could promote SC proliferation and apoptosis, down regulation of cytokines and Smad2, 4. Altered expressions of TGF-beta 1 may affect Smad and AKT but not c-Jun and extracellular regulated protein kinase (ERK) pathways. Our results revealed the role of TGF-beta 1 on WD and provided the basis for the molecular mechanisms of TGF-beta 1-regulated nerve degeneration and/or regeneration. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.