Infection of swine with Mycobacterium bovis as a model of human tuberculosis
Infection of swine with Mycobacterium bovis as a model of human tuberculosis
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DOI：
10.1086/514155
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发表时间：
1997-12-01
影响因子：
6.4
通讯作者：
Molitor, TW
中科院分区：
文献类型：
--
作者：
Bolin, CA;Whipple, DL;Molitor, TW
用牛分枝杆菌感染猪建立人肺结核和播散性结核模型。牛通过静脉内（iv）、小肠内（int）或扁桃体途径。接种后17 - 60天对动物实施安乐死，并采集组织进行培养和组织病理学检查。在给予10（4）或10（8）cfu M的猪中发现播散性结核病病变。iv或int;在给予10（2）或10（3）cfu M的猪中发现局部肺部疾病。病变范围从具有凝固性坏死、上皮样巨噬细胞和纤维化的组织良好的结节到具有凝固性坏死和M细胞外生长的大的膨胀结节。牛，在给予M. bovis iv，感染M.牛是用于阐明人类结核病发病机制和宿主防御机制的有用动物模型。
Swine were infected with Mycobacterium bovis to develop a model for pulmonary and disseminated tuberculosis in humans, Pigs were inoculated with various doses of M. bovis by intravenous (iv), intratracheal (int), or tonsillar routes. Animals were euthanized between 17 and 60 days after inoculation, and tissues were collected for culture and histopathologic examination. Lesions of disseminated tuberculosis were found in pigs given 10(4) or 10(8) cfu of M. bovis iv or int; localized pulmonary disease was found in pigs given 10(2) or 10(3) cfu of M. bovis int. Lesions ranged from well-organized tubercles with coagulative necrosis, epithelioid macrophages, and fibrosis to large expansive tubercles with liquefactive necrosis and extracellular growth of M. bovis, Tuberculous meningitis was observed in animals given M. bovis iv, Swine infected with M. bovis are a useful animal model for elucidating the mechanisms of pathogenesis and host defense to tuberculosis in humans.