Primary dendritic cells phagocytose Cryptococcus neoformans via mannose receptors and Fcγ receptor II for presentation to T lymphocytes
Primary dendritic cells phagocytose Cryptococcus neoformans via mannose receptors and Fcγ receptor II for presentation to T lymphocytes
复制标题
DOI：
10.1128/iai.70.11.5972-5981.2002
复制
发表时间：
2002-11-01
影响因子：
3.1
通讯作者：
Mody, CH
中科院分区：
文献类型：
--
作者：
Syme, RM;Spurrell, JCL;Mody, CH
不同的“专业”抗原呈递细胞（APC）具有独特的特性，根据生物体的不同，有利于或限制微生物抗原向T细胞的呈递。新型隐球菌是一种致病性酵母，对APC提出了独特的挑战，包括其大尺寸，其刚性细胞壁以及其刺激T细胞作为有丝分裂原的能力。T细胞对C.新生儿有丝分裂原（CnM）需要在将CnM呈递给T细胞之前通过辅助细胞吞噬和加工生物体。由于需要摄取的有机体和更有限的共刺激需要的有丝分裂原，巨噬细胞可能是最有可能的细胞来源的辅助细胞。然而，本研究表明，具有树突状形态的CD 3（-）、CD 14（-）、CD 19（-）、CD 56（-）、HLA-DR+和CD 83（+）的瞬时贴壁细胞，而不是单核细胞衍生的或组织（肺泡）巨噬细胞，是呈递CnM的最有效APC。大量的这些细胞结合并内化生物体，并且仅需要少量的树突状细胞来呈递有丝分裂原。T细胞。此外，甘露糖受体和Fc γ受体II是C.因为阻断这些受体中的任何一种都会废除对C.新生儿和淋巴细胞增殖响应于CnM。这些研究证明了令人惊讶的事实，树突状细胞是CnM最有效的辅助细胞。
Different "professional" antigen-presenting cells (APC) have unique characteristics that favor or restrict presentation of microbial antigens to T cells, depending on the organism. Cryptococcus neoformans is a pathogenic yeast that presents unique challenges to APC, including its large size, its rigid cell wall, and its ability to stimulate T cells as a mitogen. T-cell proliferation in response to the C. neoformans mitogen (CnM) requires phagocytosis and processing of the organisms by accessory cells prior to presentation of CnM to T cells. Because of the requirement for uptake of the organism and more limited costimulatory requirements of mitogens, macrophages might be the most likely cellular source for the accessory cell. However, the present study demonstrates that a transiently adherent cell that was CD3(-), CD14(-), CD19(-), CD56(-), HLA-DR+, and CD83(+) with a dendritic morphology, rather than monocyte-derived or tissue (alveolar) macrophages, was the most efficient APC for presentation of CnM. A large number of these cells bound and internalized the organism, and only a small number of dendritic cells were required for presentation of the mitogen to. T cells. Further, the mannose receptor and Fcgamma receptor II were required for presentation of C. neoformans, as blocking either of these receptors abrogated both uptake of C. neoformans and lymphocyte proliferation in response to CnM. These studies demonstrate the surprising fact that dendritic cells are the most efficient accessory cells for CnM.