Doxorubicin-loaded poly (lactic-co-glycolic acid) nanoparticles coated with chitosan/alginate by layer by layer technology for antitumor applications.
Doxorubicin-loaded poly (lactic-co-glycolic acid) nanoparticles coated with chitosan/alginate by layer by layer technology for antitumor applications.
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负载阿霉素的聚（乳酸-乙醇酸）纳米颗粒通过逐层技术涂覆壳聚糖/海藻酸盐，用于抗肿瘤应用
DOI：
10.2147/ijn.s130404
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发表时间：
2017
影响因子：
8
通讯作者：
Zheng C
中科院分区：
文献类型：
--
作者：
Chai F;Sun L;He X;Li J;Liu Y;Xiong F;Ge L;Webster TJ;Zheng C
将壳聚糖(CHI)和海藻酸盐(ALG)交替沉积在阿霉素(DOX)载药的聚乳酸-乙醇酸(PLGA)纳米粒(NPs)上，通过层层自组装控制药物的释放。研究了聚电解质浓度、氯化钠浓度、温度等因素对纳米胶体颗粒多层膜生长的影响。阐明了CHI/ALG多层膜的生长机制。用透射电子显微镜、原子力显微镜、X射线衍射仪和Zeta电位分析仪对包覆后的纳米粒子进行了表征。体外研究表明，DOX-PLGA纳米粒(DOX-PLGA纳米粒)在体外存在不良的突释现象，通过逐层释放技术，其释放率从55.12%降低到5.78%。当介质pH值从7.4降至5.0时，DOX的释放量增加了40%以上。更重要的是，与DOX-PLGA(CHI/ALG)3纳米粒和DOX溶液相比，DOX-PLGA(CHI/ALG)3纳米粒具有更高的体内抑瘤率83.17%和更低的毒性。因此，目前制备的PLGA-聚电解质纳米粒在许多抗癌药物控制释放方面具有很强的潜在应用。
Natural polyelectrolyte multilayers of chitosan (CHI) and alginate (ALG) were alternately deposited on doxorubicin (DOX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) with layer by layer self-assembly to control drug release for antitumor activity. Numerous factors which influenced the multilayer growth on nano-colloidal particles were studied: polyelectrolyte concentration, NaCl concentration and temperature. Then the growth regime of the CHI/ALG multilayers was elucidated. The coated NPs were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction and a zeta potential analyzer. In vitro studies demonstrated an undesirable initial burst release of DOX-loaded PLGA NPs (DOX-PLGA NPs), which was relieved from 55.12% to 5.78% through the use of the layer by layer technique. The release of DOX increased more than 40% as the pH of media decreased from 7.4 to 5.0. More importantly, DOX-PLGA (CHI/ALG)3 NPs had superior in vivo tumor inhibition rates at 83.17% and decreased toxicity, compared with DOX-PLGA NPs and DOX in solution. Thus, the presently formulated PLGA-polyelectrolyte NPs have strong potential applications for numerous controlled anticancer drug release applications.