Indirect Detection of Glycosidases Using Amperometry.
Indirect Detection of Glycosidases Using Amperometry.
复制标题
DOI：
10.1021/acs.analchem.5b03943
复制
发表时间：
2016-03
影响因子：
7.4
通讯作者：
B. P. Gurale;A. Dhawane;Xikai Cui;Amrita Das;Xiaohu Zhang;S. Iyer
中科院分区：
文献类型：
--
作者：
B. P. Gurale;A. Dhawane;Xikai Cui;Amrita Das;Xiaohu Zhang;S. Iyer
糖苷酶是分解糖苷键的基本酶。糖苷酶的存在已被广泛用于检测病原体、标记细胞/组织和报告特定疾病。我们开发了一种快速电化学检测糖苷酶的方法。将不具有电化学活性的底物暴露在糖苷酶下释放葡萄糖，这可以很容易地使用电化学电池进行测量。5种不同的糖苷酶用一次性电极在1 h内快速检测。该试验可以很容易地结合到重新使用的血糖仪中，以快速检测糖苷酶，这反过来又可以用于报告病原体或疾病的存在。
Glycosidases are essential enzymes that cleave glycoside bonds. The presence of glycosidases have been widely used to detect pathogens, label cells/tissues, and report specific diseases. We have developed a rapid electrochemical assay to detect glycosidases. Exposure of electrochemically inactive substrates to glycosidases releases glucose, which can be measured easily using an electrochemical cell. Five different glycosidases were detected rapidly within 1 h using disposable electrodes. This assay could readily be incorporated into repurposed glucose meters to rapidly detect glycosidases, which in turn could be useful to report the presence of a pathogen or illness.