Production of Acinetobacter radioresistens lipase using Tween 80 as the carbon source
Production of Acinetobacter radioresistens lipase using Tween 80 as the carbon source
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DOI：
10.1016/s0141-0229(01)00396-9
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发表时间：
2001-09-05
影响因子：
3.4
通讯作者：
Chen, TL
中科院分区：
文献类型：
--
作者：
Li, CY;Cheng, CY;Chen, TL
以Tween 80为碳源，对耐辐射不动杆菌产脂肪酶进行了研究.与橄榄油相比，吐温80在发酵液中的水解速度要慢得多。吐温80利用其缓慢的水解速率，通过控制释放的方式为细胞生长和脂肪酶的合成提供油酸，避免了油酸对脂肪酶合成的抑制作用。在2.5L罐发酵罐中，当添加0.3%（v/v）吐温80时，发酵时间为6 h，脂肪酶产量为25 U/mL。吐温80浓度的最佳产率的存在是细胞生长和抑制脂肪酶合成之间的妥协的结果。为进一步提高脂肪酶的产率，采用pH-stat补料分批发酵法，在16 h内达到120 U/mL。生产A.抗辐射脂肪酶进行比较。(C)2001 Elsevier Science Inc. All rights reserved.
Lipase of Acinetobacter radioresistens was produced using Tween 80 as the carbon source. Compared with olive oil, the hydrolysis rate of Tween 80 in the fermentation broth was much slower. Taking advantage of the slow hydrolysis rate, Tween 80 could provide oleic acid for cell growth and lipase production through a mode of controlled release, and the known repression of lipase synthesis by oleic acid could be avoided. The optimal lipase yield obtained with batch culture in a 2.5-L tank fermentor was 25 U/mL with a fermentation time of 6 h, when using 0.3% (v/v) Tween 80. The existence of an optimal yield with respect to concentration of Tween 80 was a result of the compromise between cell growth and the repression of lipase synthesis. To further the lipase productivity, a fed-batch culture with the pH-stat feeding method was employed, and the lipase productivity achieved was 120 U/mL in 16 h. Various strategies for production of A. radioresistens lipase were compared. (C) 2001 Elsevier Science Inc. All rights reserved.