Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.
Alpha 2-macroglobulin 'fast' forms inhibit superoxide production by activated macrophages.
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α2-巨球蛋白“快速”形式抑制活化巨噬细胞产生超氧化物。
DOI：
10.1016/0304-4165(83)90384-7
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Pizzo,SV
中科院分区：
文献类型：
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作者：
Hoffman,M;Feldman,SR;Pizzo,SV
用卡介苗激活的小鼠腹腔巨噬细胞与人α-2-巨球蛋白孵育，用胰酶或甲胺转化为“快速”形式，然后用佛波酯刺激巨噬细胞。α-2-巨球蛋白-胰酶和α-2-巨球蛋白-甲胺均能抑制巨噬细胞产生超氧阴离子(O2-−)，而天然α-2-巨球蛋白只有在高浓度时才有抑制作用。α-2-巨球蛋白“快速”形式与约8 nM的AKdof结合，在7 nM的浓度下抑制50%O2−(ID_(50))的产生，而α-2-巨球蛋白则抑制O2-−的产生，ID_(50)为141 nM。α-2-巨球蛋白的“快速”形式可能在炎症反应的反馈调节中发挥作用。
Mouse peritoneal macrophages activated by bacillus Calmette-Guerin (BCG) were incubated with humanα2-macroglobulin converted to its ‘fast’ form with either trypsin or methylamine before being stimulated with phorbol myrystate acetate. Bothα2-macroglobulin-trypsin andα2-macroglobulin-methylamine inhibited macrophage production of superoxide anion (O2−) while nativeα2-macroglobulin had little effect except at high concentration. Theα2-macroglobulin ‘fast’ forms, which bind with aKdof about 8 nM, inhibited 50% generation of O2−(ID50) at a concentration of 7 nM whileα2-macroglobulin inhibited O2−production with an ID50of 141 nM. The ‘fast’ forms ofα2-macroglobulin may play a role in the feedback regulation of inflammatory reactions.