Strategies for testing long-term transcutaneous amperometric glucose sensors.
Strategies for testing long-term transcutaneous amperometric glucose sensors.
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DOI：
10.1089/dia.2005.7.927
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发表时间：
2005-12-01
影响因子：
5.4
通讯作者：
Moussy, Francis
中科院分区：
文献类型：
--
作者：
Long, Nathan;Yu, Bazhang;Moussy, Francis
目的：开发了经皮和嵌入式器械，用于表征皮下植入葡萄糖传感器的体内性能。这些器械用作从外部访问电化学葡萄糖传感器的入口。它们的设计旨在防止传感器从动物体内拔出和传感器导线断裂。该设备的开发考虑到啮齿动物的流动性，感染控制，和动物福利平衡传感器的耐用性，可访问性，和functionals.METHODS：我们的方法是开发了五个动物协议跨越6个月的时间。在22只Sprague-Dawley远系大鼠中植入68个传感器和60个相关装置。结果：传感器的平均寿命为11.2 ± 3.1天，最长为56天。全因传感器故障平均每天一个传感器。随着植入器械的改进，可归因于器械的失效减少了40%。由此产生的设备表现出良好的耐用性，并允许方便的传感器访问和testing.CONCLUSIONS：这些数据代表基线传感器的功能，对未来的传感器的改进可以测量。所描述的新设备和技术应该是开发连续葡萄糖传感器的有价值的工具。
OBJECTIVES: Transcutaneous and embedded devices were developed for use in characterizing the in vivo performance of subcutaneously implanted glucose sensors. The devices were used as a portal for accessing electrochemical glucose sensors from the exterior. They were designed to prevent the sensors from being pulled out of the animals and the sensor leads from breaking. Development of the devices took into consideration rodent mobility, infection control, and animal welfare balanced with sensor durability, accessibility, and functionality.METHODS: Our approach was developed over five animal protocols spanning a period of 6 months. A total of 68 sensors were implanted with 60 associated devices in 22 Sprague-Dawley outbred rats.RESULTS: The average sensor lifetime was 11.2 +/- 3.1 days with a maximum of 56 days. All-cause sensor failure averaged one sensor per day. As implantation devices were modified, failure attributable to the device was decreased by 40%. The resulting devices showed good durability and allowed for easy sensor access and testing.CONCLUSIONS: These data represent baseline sensor function against which future sensor improvements may be measured. The new devices and techniques described should be a valuable tool in the development of continuous glucose sensors.