Chorioallantoic placenta defects in cloned mice.
Chorioallantoic placenta defects in cloned mice.
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DOI：
10.1016/j.bbrc.2006.08.057
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发表时间：
2006-10
影响因子：
3.1
通讯作者：
N. Wakisaka-Saito;T. Kohda;K. Inoue;N. Ogonuki;H. Miki;Takafusa Hikichi;E. Mizutani;T. Wakayama;T. Kaneko-Ishino;A. Ogura;F. Ishino
中科院分区：
文献类型：
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作者：
N. Wakisaka-Saito;T. Kohda;K. Inoue;N. Ogonuki;H. Miki;Takafusa Hikichi;E. Mizutani;T. Wakayama;T. Kaneko-Ishino;A. Ogura;F. Ishino
体细胞核移植技术已在多种哺乳动物中成功地产生活体克隆，但成功率很低。在小鼠中，大约一半的核移植胚胎会植入，但很少能存活到足月。我们对妊娠 10.5 dpc 时卵丘细胞产生的克隆小鼠胚胎的胎盘进行了详细的组织学分析，在这个阶段大多数克隆已经终止发育。在 10.5 dpc 时，胚外组织表现出几种明确的组织学模式，每种模式都反映了它们的发育停滞阶段。最显着的异常是二倍体细胞的海绵滋养层发育不良。这与在 12.5 dpc 或更高阶段的体细胞克隆中经常观察到的胎盘增生形成对比。在胚胎中还观察到了多种结构异常。胎盘和胚胎缺陷可能导致小鼠克隆成功率低。
Somatic cell nuclear transfer technology has been applied to produce live clones successfully in several mammalian species, but the success rates are very low. In mice, about half of the nuclear transfer embryos undergo implantation, but very few survive to term. We undertook detailed histological analyses of placentas from cloned mouse embryos generated from cumulus cells at 10.5 dpc of pregnancy, by which stage most clones have terminated their development. At 10.5 dpc, the extraembryonic tissues displayed several defined histological patterns, each reflecting their stage of developmental arrest. The most notable abnormality was the poor development of the spongiotrophoblast layer of diploid cells. This is in contrast to the placental hyperplasia frequently observed in somatic clones at 12.5 dpc or later stages. A variety of structural abnormalities were also observed in the embryos. Both placental and embryonic defects likely contribute to the low success rate of the mouse clones.