FURTHER INSIGHTS INTO MALUS FUSCA FIRE BLIGHT RESISTANCE
FURTHER INSIGHTS INTO MALUS FUSCA FIRE BLIGHT RESISTANCE
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DOI：
10.4454/jpp.v99i0.3908
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Peil, A.
中科院分区：
文献类型：
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作者：
Emeriewen, O. F.;Richter, K.;Peil, A.
苹果野生种Malus fusca MAL0045在人工接种淀粉状欧文氏菌Ea222_JKI的试验中，发现其对火疫病具有抗性。因此，利用MAL0045与Idared杂交得到的群体，在10号染色体上定位了fusca M. Mfu10的相应抗火疫病位点，解释了高达66%的表型变异，其与菌株Ea222_JKI的奇数（LOD）比为31.0。此外，极具侵袭性的菌株Ea3049对MAL0045的影响很小，但对种群的影响很大，虽然不能克服Mfu10的抗性。为了进一步了解fusca的抗性机制，我们对134个原始定位群体进行了对E. amylovora菌株ZYRKD3-1的抗性评估，该菌株对M.xrobusta 5 （Mr5）和Mr5在LG3上的抗火疫病QTL的抗性破坏。结果表明，虽然ZYRKD3-1对Mfu10的平均病斑百分数比Ea222_JKI提高了近一倍，但在LG10上仍能检测到fusca主QTL，且对抗火枯病的影响较高，表明ZYRKD3-1对Mfu10没有影响。
The apple wild species accession Malus fusca MAL0045 had been found to be resistant to fire blight in artificial inoculation trials with Erwinia amylovora strain Ea222_JKI. Consequently, using a population derived by crossing MAL0045 with Idared, the corresponding fire blight resistance locus of M. fusca (Mfu10) was mapped on chromosome 10 explaining up to 66% of phenotypic variation at a logarithm of the odd (LOD) ratio of 31.0 with the strain Ea222_JKI. Moreover, the very aggressive strain Ea3049 only minimally affected MAL0045 but significantly affected the population although could not overcome the resistance of Mfu10. To further understand the resistance mechanism of M. fusca, we evaluated resistance of the original mapping population, comprised of 134 individuals, to E. amylovora strain ZYRKD3-1 which causes the breakdown of the resistance of M.xrobusta 5 (Mr5) and the Mr5 fire blight resistance QTL on LG3. Our results showed that the major QTL of M. fusca on LG10 could still be detected at the same exact position with a higher effect on fire blight resistance, indicating that ZYRKD3-1 has no effect on Mfu10, although the mean percent lesion length of the population was almost doubled compared with Ea222_JKI.