Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis
Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis
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DOI：
10.1016/s8756-3282(01)00612-3
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发表时间：
2001-12-01
期刊：
影响因子：
4.1
通讯作者：
Gronthos, S
中科院分区：
文献类型：
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作者：
Shi, S;Robey, PG;Gronthos, S
我们比较了人牙髓干细胞（DPSCs）和骨髓基质干细胞（BMSSCs）的基因表达谱作为牙祖细胞和骨祖细胞的代表性群体，分别。将来自原代培养物的总RNA逆转录以产生cDNA探针，然后与Research Genetics人基因微阵列过滤器GF 211杂交。使用PATHWAYS软件包分析微阵列。发现人类DPSC和BMSSC对于超过4000个已知的人类基因具有相似的基因表达水平。一些差异表达的基因，包括胶原蛋白XVIII α 1、胰岛素样生长因子-2（IGF-2）、discordin结构域酪氨酸激酶2、NAD（P）H甲萘醌氧化还原酶、果蝇大碟同源物2和细胞周期蛋白依赖性激酶6在DPSC中高度表达，而胰岛素样生长因子结合蛋白-7（IGFBP-7）和胶原蛋白I α 2在BMSSC中更高表达。此外，我们证实了这些基因的差异表达的半定量聚合酶链反应（PCR）和北方印迹杂交。IGF-2和IGFBP-7的蛋白表达模式与DPSC和BMSSC之间观察到的差异mRNA水平相关。本报告描述了两个不同的前体人口与矿化组织的基因表达模式，并提供了进一步表征的功能作用，为许多这些基因在牙本质和骨的发展的基础。(C)2001年，Elsevier Science Inc. All rights reserved.
We compared the gene expression profiles of human dental pulp stern cells (DPSCs) and bone marrow stromal stern cells (BMSSCs) as representative populations of odontoprogenitor and osteoprogenitor cells, respectively. Total RNA from primary cultures was reverse-transcribed to generate cDNA probes and then hybridized with the Research Genetics human gene microarray filter GF211. The microarrays were analyzed using the PATHWAYS software package. Human DPSCs and BMSSCs were found to have a similar level of gene expression for more than 4000 known human genes. A few differentially expressed genes, including collagen type XVIII alpha1, insulin-like growth factor-2 (IGF-2), discordin domain tyrosine kinase 2, NAD(P)H menadione oxidoreductase, homolog 2 of Drosophila large disk, and cyclin-dependent kinase 6 were highly expressed in DPSCs, whereas insulin-like growth factor binding protein-7 (IGFBP-7), and collagen type I alpha2 were more highly expressed in BMSSCs. Furthermore, we confirmed the differential expression of these genes by semiquantitative polymerase chain reaction (PCR) and northern blot hybridization. The protein expression patterns for both IGF-2 and IGFBP-7 correlated with the differential mRNA levels seen between DPSCs and BMSSCs. This report describes the gene expression patterns of two distinct precursor populations associated with mineralized tissue, and provides a basis for further characterization of the functional roles for many of these genes in the development of dentin and bone. (C) 2001 by Elsevier Science Inc. All rights reserved.