Co-ordinated Regulations of mRNA Synthesis and Decay during Cold Acclimation in Arabidopsis Cells
Co-ordinated Regulations of mRNA Synthesis and Decay during Cold Acclimation in Arabidopsis Cells
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DOI：
10.1093/pcp/pcx059
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发表时间：
2017-06-01
影响因子：
4.9
通讯作者：
Chiba, Yukako
中科院分区：
文献类型：
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作者：
Arae, Toshihiro;Isai, Shiori;Chiba, Yukako
植物具有冷驯化系统，通过预暴露于非冻结的低温来获得抗冻性。C-repeat-binding factors（CBFs）/dehydration response element-binding factors（DREBs）的转录级联反应被认为是冷驯化过程中主要的转录调控途径。然而，关于mRNA稳定性调节在基因表达对冷胁迫的响应中的功能意义知之甚少。单个mRNA的实际水平由mRNA合成和降解之间的平衡决定。因此，重要的是要评估的调控步骤，以增加我们对基因调控的理解。在这里，我们分析了mRNA的量和半衰期的时间变化，在拟南芥细胞培养的基础上，全基因组分析的冷胁迫。在这种mRNA衰变阵列方法中，mRNA半衰期测量和微阵列分析相结合。此外，转录率的积分值的时间变化估计从上述两个参数使用的数学方法。我们的研究结果表明，几个冷响应基因，包括冷调节15 a，相对不稳定，而mRNA的量增加，在冷处理过程中，通过加快转录速率，以克服不稳定。考虑到mRNA合成和降解的动力学，这一明显矛盾的结果支持mRNA不稳定有利于CBF响应基因响应冷应激的迅速增加。
Plants possess a cold acclimation system to acquire freezing tolerance through pre-exposure to non-freezing low temperatures. The transcriptional cascade of C-repeat-binding factors (CBFs)/dehydration response element-binding factors (DREBs) is considered a major transcriptional regulatory pathway during cold acclimation. However, little is known regarding the functional significance of mRNA stability regulation in the response of gene expression to cold stress. The actual level of individual mRNAs is determined by a balance between mRNA synthesis and degradation. Therefore, it is important to assess the regulatory steps to increase our understanding of gene regulation. Here, we analyzed temporal changes in mRNA amounts and half-lives in response to cold stress in Arabidopsis cell cultures based on genome-wide analysis. In this mRNA decay array method, mRNA half-life measurements and microarray analyses were combined. In addition, temporal changes in the integrated value of transcription rates were estimated from the above two parameters using a mathematical approach. Our results showed that several cold-responsive genes, including Cold-regulated 15a, were relatively destabilized, whereas the mRNA amounts were increased during cold treatment by accelerating the transcription rate to overcome the destabilization. Considering the kinetics of mRNA synthesis and degradation, this apparently contradictory result supports that mRNA destabilization is advantageous for the swift increase in CBF-responsive genes in response to cold stress.