REGULATION AND EXPRESSION OF A GROWTH ARREST-SPECIFIC GENE (GAS5) DURING GROWTH, DIFFERENTIATION, AND DEVELOPMENT
REGULATION AND EXPRESSION OF A GROWTH ARREST-SPECIFIC GENE (GAS5) DURING GROWTH, DIFFERENTIATION, AND DEVELOPMENT
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DOI：
10.1128/mcb.12.8.3514
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发表时间：
1992-08-01
影响因子：
5.3
通讯作者：
SORRENTINO, V
中科院分区：
文献类型：
--
作者：
COCCIA, EM;CICALA, C;SORRENTINO, V
从小鼠基因组DNA中分离出生长停滞特异性gas5基因，并对其结构进行了表征。 转录单位分为12个外显子，跨度约为7 kb。 选择性剪接机制产生两种成熟mRNA，其含有11或12个外显子，并且两者都存在于生长停滞细胞的细胞质中。 在体内，gas5基因在发育和成年期的小鼠组织中广泛表达。 在Friend白血病和NIH 3T3细胞中，饱和密度停滞细胞中gas5基因mRNA的水平较高，而在活跃生长的细胞中几乎检测不到。 连续实验表明，在生长和停滞细胞中，gas5基因的转录水平相同。 另一方面，在二甲基亚砜诱导的分化细胞中，在细胞达到有丝分裂后阶段之前不久，观察到转录速率急剧下降。 这些结果表明，在密度逮捕细胞的积累的气体5 mRNA的转录后水平控制，而在分化细胞的表达调节转录。
The growth arrest-specific gas5 gene was isolated from mouse genomic DNA and structurally characterized. The transcriptional unit is divided into 12 exons that span around 7 kb. An alternative splicing mechanism gives rise to two mature mRNAs which contain either 11 or 12 exons, and both are found in the cytoplasm of growth-arrested cells. In vivo, the gas5 gene is ubiquitously expressed in mouse tissues during development and adult life. In Friend leukemia and NIH 3T3 cells, the levels of gas5 gene mRNA were high in saturation density-arrested cells and almost undetectable in actively growing cells. Run-on experiments indicated that the gas5 gene is transcribed at the same level in both growing and arrested cells. On the other hand, in dimethyl sulfoxide-induced differentiating cells a sharp decrease in the rate of transcription was observed shortly before the cells reached the postmitotic stage. These results indicate that in density-arrested cells accumulation of gas5 mRNA is controlled at the posttranscriptional level while in differentiating cells expression is regulated transcriptionally.