Association of Putative Fungal Effectors in Fusarium oxysporum with Wilt Symptoms in Soybean
Association of Putative Fungal Effectors in Fusarium oxysporum with Wilt Symptoms in Soybean
复制标题
DOI：
10.1094/phyto-11-15-0293-r
复制
发表时间：
2016-07-01
期刊：
影响因子：
3.2
通讯作者：
Munkvold, Gary P.
中科院分区：
文献类型：
--
作者：
Ellis, Margaret L.;Lanubile, Alessandra;Munkvold, Gary P.
尖孢镰刀菌复合体中的真菌可引起大豆根腐病、苗枯病和枯萎病。从大豆中回收的分离株的攻击性及其产生的症状类型各不相同。本研究的目的是鉴定遗传标记来检测侵袭性大豆枯萎病菌株。主要从大豆中收集的 80 个分离株在温室中使用易受影响的“杰克”大豆测试了它们产生枯萎症状的能力。对相同的 80 个分离株进行了真菌效应基因的存在评估。 Fmk1、Fowl、Pda1、PelA、PelD、Pep1、Prt1、Rho1、Sge1、Six1、Six6 和 Snf1。对所有聚合酶链式反应扩增子进行了测序，推断了系统发育，并对 12 个基因中的 10 个进行了分子方差分析 (AMOVA)。 3 个分离株观察到根或茎维管束变色的高发生率，而 25 个分离株观察到中度至低水平的发生率。真菌效应基因 Fmk1、Fow1、PelA、Rho1、Sge1 和 Snf1 存在于所有筛选的分离株中，而 Pdal1、PelD、Pep1、Prt1、Six1 和 Six6 分散在分离株中。贝叶斯和 AMOVA 分析发现，基因 Fmk1、Fow1、Pda1、PelA、Rho1、Sge1 和 Snf1 对应于先前基于 tef1 α 和线粒体小亚基序列指定的进化枝。这些基因均与大豆枯萎症状没有显着关联。有趣的是，Six6 基因仅存在于三种先前已知的大豆、菜豆和番茄枯萎病分离株中。其中，在我们的研究中，大豆和菜豆分离物产生了高水平的维管束枯萎病。
Fungi within the Fusarium oxysporum species complex can cause root rot, seedling blight, and wilt of soybean. Isolates recovered from soybean vary in aggressiveness and also the type of symptoms they produce. The aim of this study was to identify genetic markers to detect aggressive soybean wilt isolates. Eighty isolates collected primarily from soybean were tested in the greenhouse for their ability to produce wilt symptoms using susceptible 'Jack' soybean. The same 80 isolates were assessed for the presence of fungal effector genes. Fmk1, Fowl, Pda1, PelA, PelD, Pep1, Prt1, Rho1, Sge1, Six1, Six6, and Snf1. All polymerase chain reaction amplicons were sequenced, phylogenies were inferred, and analysis of molecular variance (AMOVA) was performed for 10 of the 12 genes. High incidence of vascular discoloration of roots or stems was observed with 3 isolates, while moderate to low levels of incidence were observed for 25 isolates. Fungal effector genes Fmk1, Fow1, PelA, Rho1, Sge1, and Snf1 were present in all isolates screened, while Pdal1, PelD, Pep1, Prt1, Six1, and Six6 were dispersed among isolates. The Bayesian and AMOVA analyses found that the genes Fmk1, Fow1, Pda1, PelA, Rho1, Sge1, and Snf1 corresponded to previously designated clades based on tef1 alpha and mitochondrial small subunit sequences. None of the genes had a significant association with wilt symptoms on soybean. Interestingly, the Six6 gene was only present in three previously known wilt isolates from soybean, common bean, and tomato; of these, the soybean and common bean isolates produced high levels of vascular wilt in our study.