Proteomic analysis of global changes in protein expression during bile salt exposure of Bifidobacterium longum NCIMB 8809
Proteomic analysis of global changes in protein expression during bile salt exposure of Bifidobacterium longum NCIMB 8809
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DOI：
10.1128/jb.187.16.5799-5808.2005
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发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Zagorec, M
中科院分区：
文献类型：
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作者：
Sánchez, B;Champomier-Vergès, MC;Zagorec, M
对胆汁应激的适应和耐受是确保双歧杆菌在人类肠道环境中存活的主要限制因素之一。研究了胆盐对长双歧杆菌蛋白质表达模式的影响。与胆汁提取物或不与胆汁提取物一起生长的菌株的蛋白质模式比较使我们能够鉴定出34种不同的蛋白质，其表达受到调节。这些蛋白质中的大多数在少量（0.6 g/L（-1））和大量（1.2 g/L（-1））暴露于胆汁后被诱导。这些包括一般应激反应分子伴侣，参与转录和翻译以及氨基酸和核苷酸代谢的蛋白质，以及糖酵解和丙酮酸催化剂的几种酶。值得注意的是，木酮糖5-磷酸/果糖6-磷酸转酮酶，所谓的双歧杆菌细菌分流的关键酶，被发现是上调，和果糖6-磷酸的活性显着较高的蛋白质提取物的细胞生长在胆汁的存在下。还检测到代谢终产物（乙酸盐和乳酸盐）水平的变化。这些结果表明，胆汁盐，其中双歧杆菌自然暴露，诱导一个复杂的生理反应，而不是一个单一的事件，其中蛋白质从许多不同的功能类别参加。这项研究扩展了我们对肠道双歧杆菌耐受胆汁能力的分子机制的理解。
Adaptation to and tolerance of bile stress are among the main limiting factors to ensure survival of bifidobacteria in the intestinal environment of humans. The effect of bile salts on protein expression patterns of Bifidobacterium longum was examined. Protein pattern comparison of strains grown with or without bile extract allowed us to identify 34 different proteins whose expression was regulated. The majority of these proteins were induced after both a minor (0.6 g liter(-1)) and a major (1.2 g liter(-1)) exposure to bile. These include general stress response chaperones, proteins involved in transcription and translation and in the metabolism of amino acids and nucleotides, and several enzymes of glycolysis and pyruvate catabolism. Remarkably, xylulose 5-phosphate/fructose 6-phosphate phosphoketolase, the key enzyme of the so-called bifidobacterial shunt, was found to be upregulated, and the activity on fructose 6-phosphate was significantly higher for protein extracts of cells grown in the presence of bile. Changes in the levels of metabolic end products (acetate and lactate) were also detected. These results suggest that bile salts, to which bifidobacteria are naturally exposed, induce a complex physiological response rather than a single event in which proteins from many different functional categories take part. This study has extended our understanding of the molecular mechanism underlying the capacity of intestinal bifidobacteria to tolerate bile.