Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing.
Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing.
复制标题
人类醛缩酶A基因。
DOI：
10.1111/j.1432-1033.1988.tb14136.x
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
F. Salvatore
中科院分区：
文献类型：
--
作者：
P. Izzo;P. Costanzo;A. Lupo;E. Rippa;G. Paolella;F. Salvatore
报告了人醛缩酶 A 同工酶基因的完整核苷酸序列。克隆的基因序列横跨 7530 bp，包括 12 个外显子，每个单倍体人类基因组都有一个拷贝。该基因的结构组织相当复杂：含有编码序列的八个外显子是从人类和其他哺乳动物来源提取的所有 mRNA 所共有的； 5'非翻译区存在四个额外的外显子，其中一个包含在普遍存在的 mRNA 类型中，第二个包含在肌肉特异性类型的 mRNA 中，第三个和第四个外显子包含在人类肝脏组织中发现的少数种类的 mRNA 中。此外，确定的序列包括5'侧翼区中第一外显子(外显子I)上游的1000个核苷酸，以及终止密码子下游的400个核苷酸，其中包括多聚腺苷酸化信号。对从人类培养的成纤维细胞、肌肉和肝癌细胞系中提取的 mRNA 5' 端进行 S1 核酸酶保护分析表明存在四个不同的转录起始位点。后者也得到了真核启动子常规序列的支持。因此，同一基因上的四个启动子产生不同的组织特异性转录本，它们共享翻译序列，但由于差异mRNA加工，每个启动子都有一个独特的5'非翻译区。三个人类和哺乳动物醛缩酶A、B和C基因的编码区和内含子-外显子组织的核苷酸同源性证实它们源自共同的祖先基因，并且醛缩酶B首先分化。
The complete nucleotide sequence of the human aldolase A isoenzyme gene is reported. The cloned gene sequence, spanning 7530 bp, includes twelve exons and occurs as a single copy per haploid human genome. The structural organization of the gene is quite complex: eight exons containing the coding sequence are common to all mRNAs extracted from human and other mammalian sources; four additional exons are present in the 5' untranslated region, of these one is contained in the ubiquitous type of mRNA, the second is in the muscle-specific type of mRNA and the third and fourth are in a minor species of mRNA found in human liver tissue. Furthermore, the determined sequence includes 1000 nucleotides upstream from the first exon (exon I) in the 5' flanking region, and 400 nucleotides, which include the polyadenylation signal, downstream from the termination codon. S1-nuclease-protection analysis of the 5' end of mRNA extracted from human cultured fibroblasts, muscle and hepatoma cell lines indicates the existence of four different transcription-initiation sites. The latter are also supported by the presence of conventional sequences for eukaryotic promoters. Therefore, the four promoters on the same gene generate different tissue-specific transcripts, which share the translated sequence, but each has a unique 5' untranslated region as a result of differential mRNA processing. The nucleotide homology at the coding region and the intron-exon organization of the three human and mammalian aldolase A, B and C genes confirm that they arose from a common ancestral gene, and that aldolase B diverged first.
通过 5-非翻译区的多个转录起始位点和内含子剪接位点确定 3-羟基-3-甲基戊二酰辅酶 A 还原酶的多个 mRNA。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Reynolds,GA;Goldstein,JL;Brown,MS
通讯作者：
Brown,MS