Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment.
Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment.
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DOI：
10.1084/jem.160.6.1937
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发表时间：
1984-12-01
期刊：
影响因子：
--
通讯作者：
Watanabe T
中科院分区：
文献类型：
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作者：
Ishihara T;Kudo A;Watanabe T
从人浆细胞白血病细胞系ARH-77中克隆了一套完整的重排的人γ 1-重链基因，HIGH 1，并将其转移到小鼠细胞中。它在小鼠骨髓瘤细胞中强烈表达，但在小鼠L细胞中不表达，表明免疫球蛋白基因表达不是种特异性的，而是细胞特异性的。然而，一个显着的生产人γ 1链诱导小鼠L细胞含有HIGH 1基因时，细胞用放线菌酮处理一段时间。一个不稳定的阻遏分子在免疫球蛋白基因的表达中的作用提出。
A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-specific but cell-specific. However, a remarkable production of human gamma 1 chain was induced in mouse L cells containing HIG1 gene when the cells were treated with cycloheximide for a short period. The role of a labile repressor molecule in the expression of the immunoglobulin gene is proposed.