GROWTH AND DIFFERENTIATION IN THE DROSOPHILA EYE COORDINATED BY HEDGEHOG
GROWTH AND DIFFERENTIATION IN THE DROSOPHILA EYE COORDINATED BY HEDGEHOG
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DOI：
10.1038/373709a0
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发表时间：
1995-02-23
期刊：
影响因子：
64.8
通讯作者：
DONOHOE, TJ
中科院分区：
文献类型：
--
作者：
HEBERLEIN, U;SINGH, CM;DONOHOE, TJ
果蝇视网膜的分化是不同步的：它开始于眼视盘的后缘，向前发展超过2天。在此期间，椎间盘继续生长，大小增加了大约8倍。上皮上的一个凹痕，即形态发生沟(1)，标志着分化波的前沿。沟的向前发展被认为是由后眼盘中分化的感光细胞发出的信号驱动的（2,3）。这种信号的一个很好的候选者是刺猬（hh）基因的产物（4-7）；它的表达，并推测分泌，通过分化的光感受器和它的功能是需要持续的沟运动（2,3）。本研究表明，hedgehog基因的异位表达使前眼盘的异位沟发生运动，除了细胞形状的改变外，这些异位沟还与一系列紧密协调的事件有关，包括增殖、细胞周期同步和模式形成，这些事件与正常的沟进展平行。我们认为，形态发生沟与协调眼盘生长和分化的短暂边界一致，hedgehog基因是必要和充分的，可以使该边界跨越上皮。
DIFFERENTIATION Of the Drosophila retina is asynchronous: it starts at the posterior margin of the eye imaginal disc and progresses anteriorly over two days. During this time the disc continues to grow, increasing in size by approximately eightfold, An indentation in the epithelium, the morphogenetic furrow(1), marks the front edge of the differentiation wave. Anterior progression of the furrow is thought to be driven by signals emanating from differentiating photoreceptor cells in the posterior eye disc(2,3). A good candidate for such a signal is the product of the hedgehog (hh) gene(4-7); it is expressed, and presumably secreted, by differentiating photoreceptors and its function is required for continued furrow movement(2,3). Here we show that ectopic expression of hedgehog sets in motion ectopic furrows in the anterior eye disc, In addition to changes in cell shape, these ectopic furrows are associated with a tightly orchestrated series of events, including proliferation, cell cycle synchronization and pattern formation, that parallel normal furrow progression. We propose that the morphogenetic furrow coincides with a transient boundary that coordinates growth and differentiation of the eye disc, and that hedgehog is necessary and sufficient to propagate this boundary across the epithelium.