Stimulatory and inhibitory effects of adenosine A2A receptors on nerve growth factor-induced phosphorylation of extracellular regulated kinases 1/2 in PC12 cells
Stimulatory and inhibitory effects of adenosine A2A receptors on nerve growth factor-induced phosphorylation of extracellular regulated kinases 1/2 in PC12 cells
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DOI：
10.1016/s0304-3940(00)01461-0
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发表时间：
2000-10-13
影响因子：
2.5
通讯作者：
Fredholm, BB
中科院分区：
文献类型：
--
作者：
Arslan, G;Fredholm, BB
在PC 12细胞中检测了神经生长因子（NGF）、腺苷和腺苷阿萨受体激动剂（CGS 21680）对细胞外调节激酶12（ERK 12）磷酸化的影响。腺苷和CGS 21680刺激ERK 1/2，但抑制与NGF孵育10分钟诱导的ERK 1/2磷酸化。CGS 21680和NGF的较长处理（1-2小时）导致ERK 1 - 2活化的累加效应。毛喉素发挥同样的作用，表明它们是由环AMP介导的。这些结果表明，腺苷阿萨受体诱导的cAMP增加可以刺激ERK 1/2磷酸化本身，抑制和增强早期NGF诱导的ERK 1/2激活。这些结果可以解释的事实，即神经生长因子的行动是通过不同的途径介导的早期和晚期的时间点。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Effects of nerve growth factor (NGF), adenosine and an adenosine ASA receptor agonist (CGS 21680) on the phosphorylation of extracellular-regulated kinases 1/2 (ERK1/2) were examined in PC12 cells. Adenosine and CGS 21680 stimulated ERK1/2, but inhibited the phosphorylation of ERK1/2 induced by a 10 min incubation with NGF. Longer treatment with CGS 21680 and NGF (1-2 h) resulted in an additive effect on the activation of ERK1/2. Forskolin exerted the same effects, suggesting that they are mediated by cyclic AMP. These results indicate that adenosine ASA receptor induced increases in cyclic AMP can stimulate ERK1/2 phosphorylation per se, inhibit the initial and enhance the late NGF-induced activation of ERK1/2. These results may be explained by the fact that NGF action is mediated via different pathways at early and late time points. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.