Cell migration in repair of mouse corneal epithelium.
Cell migration in repair of mouse corneal epithelium.
复制标题
小鼠角膜上皮修复中的细胞迁移。
DOI：
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发表时间：
1979
影响因子：
4.4
通讯作者：
R. Buck
中科院分区：
文献类型：
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作者：
R. Buck
在小鼠体内和器官培养物中研究了通过去除上皮的全层而形成的角膜伤口的修复。直径为1 ~ 1.4 mm的伤口在12 ~ 16 h内修复完成。在这些小圆形伤口的边缘，放射状排列着伸长的上皮细胞。在大的、形状不规则的伤口中，细胞通常以其长轴与伤口边缘相切的方式排列。在这种排列的细胞上，有引导片和许多短的丝状伪足，但在放射状排列的细胞上没有。通常在边缘的凸起部分看到的上皮细胞的切线方向被认为与这些区域的前进停滞有关。周边角膜细胞参与上皮细胞的运动，这一点通过置于其中的印度墨水标记物的运动以及在与上皮细胞运动方向交叉的切口处细胞的堆积而得到证实。细胞在伤口边缘相互滚动的概念没有得到支持。它们似乎作为一个整体移动，但它们的相对位置没有显著变化。
The repair of wounds of the cornea made by removing the full thickness of epithelium was studied in mice, both in vivo and in organ cultures. Repair of wounds 1 to 1.4 mm diameter was complete in 12 to 16 hr. Elongated epithelial cells were arranged radially about the margins of these small circular wounds. In large, irregularly shaped wounds, the cells frequently lay with their long axes at a tangent to the wound margin. On cells with this arrangement, leading lamellae and numberous short filopodia were present, but they were not on the radially arranged cells. The tangential orientation of the epithelial cells, usually seen at convex parts of the margins, was believed to be associated with arrest of the advance of these areas. Participation of the cells of the peripheral cornea in the movement of the epithelium was evident by the movement of India ink markers placed in them and also by the piling up of cells at incisions made across the direction of movement of the epithelium. The concept of cells rolling over each other at the edge of the wound was not supported. They appeared to move as a mass without significant changes in their relative positions.