Effect of a bioactive curcumin derivative on DPPC membrane: A DSC and Raman spectroscopy study
Effect of a bioactive curcumin derivative on DPPC membrane: A DSC and Raman spectroscopy study
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DOI：
10.1016/j.tca.2006.03.007
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
Demetzos, Costas
中科院分区：
文献类型：
--
作者：
Gardikis, Kostantinos;Hatziantoniou, Sophia;Demetzos, Costas
研究了亲脂生物活性姜黄素衍生物二甲氧基姜黄素(1)与双棕榈酰磷脂酰胆碱（DPPC）的相互作用。采用差示扫描量热法和拉曼光谱法监测(1)引起的热力学变化及其在DPPC脂质双分子层中的位置。结果表明：(1)影响DPPC脂膜的热致性，导致预转变的消除和相变谱的扩大，并在浓度增加时略微降低t。I-2935/2880、I-2844/2880和I-1090/1130峰的拉曼高度强化比代表了(1)与烷基链的相互作用，提供了烷基链构象中无序与有序之比的信息。715 cm（-1）处的峰强度变化表明胆碱头基团与(1)相互作用。拉曼光谱分析结果与热分析结果基本一致。在开发先进的脂质载体系统（如脂质体）之前，应该研究(1)等生物活性亲脂分子与脂质双分子层的相互作用。这些研究结果为合理设计脂质给药系统提供了有关膜完整性和物理化学性质的信息。(C) 2006 Elsevier B.V.版权所有
Interactions of dimethoxycurcumin (1) a lipophilic bioactive curcumin derivative with dipalmitoyl phosphatidylcholine (DPPC) were investigated. The thermodynamic changes caused by (1) and its location into DPPC lipid bilayers were monitored by differential scanning calorimetry and Raman spectroscopy. The results reveal that (1) influences the thermotropic properties of DPPC lipid membrane causing abolition of the pretransition and broadening of the phase-transition profile and slightly decreases the T. at increasing concentrations. The Raman height intensify ratios of the peaks I-2935/2880, I-2844/2880 and I-1090/1130 are representative of the interaction of (1) with the alkyl chains and furnish information about the ratio between disorder and order that exists in the conformation of the alkyl chain. The intensity changes of the peak at 715 cm(-1) indicates interaction between the choline head group and (1). The Raman spectroscopy results are in agreement with the thermal analysis results. Biologically active lipophilic molecules such as (1) should be studied in terms of their interaction with lipid bilayers prior to the development of advanced lipid carrier systems such as liposomes. The results of these studies provide information on the membrane integrity and physicochemical properties that are essential for the rational design lipidic drug delivery systems. (C) 2006 Elsevier B.V. All rights reserved.