Hawaiian coral holobionts reveal algal and prokaryotic host specificity, intraspecific variability in bleaching resistance, and common interspecific microbial consortia modulating thermal stress responses
Hawaiian coral holobionts reveal algal and prokaryotic host specificity, intraspecific variability in bleaching resistance, and common interspecific microbial consortia modulating thermal stress responses
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夏威夷珊瑚全生物揭示了藻类和原核宿主的特异性、抗白化性的种内变异性以及调节热应激反应的常见种间微生物群落
DOI：
10.1016/j.scitotenv.2023.164040
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发表时间：
2023
影响因子：
9.8
通讯作者：
Ritson-Williams, Raphael
中科院分区：
文献类型：
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作者：
Núñez-Pons, Laura;Cunning, Ross;Nelson, Craig E.;Amend, Anthony S.;Sogin, E. Maggie;Gates, Ruth;Ritson-Williams, Raphael
从历史上看，夏威夷很少发生大规模的珊瑚白化事件，直到 2014 年至 2015 年连续发生两次热浪。在卡内奥赫湾（欧胡岛）观察到了随之而来的死亡和热应激。两种最主要的当地物种表现出抗白化性或易感性的表型二分性（Montipora capatata 和 Porites compressa），而第三个主要物种（Pocillopora acuta）对白化普遍敏感。为了调查珊瑚微生物组在白化和恢复过程中的变化，对 50 个菌落进行了标记并定期监测。对三个遗传标记（16S rRNA 基因 ITS1 和 ITS2）进行元条形码编码，然后采用用于群落结构分析、差异丰度和纵向数据相关性的组合方法来临时比较细菌/古细菌、真菌和共生科动态。 compressacorals 恢复得比 P 快。 acuta 和 Montipora headata。原核生物和藻类群落主要由宿主物种形成，并且没有明显的时间适应模式。共生藻科特征是在菌落规模上鉴定的，并且通常与漂白敏感性相关。漂白表型之间的细菌组成实际上是恒定的，并且在 P 中更加多样化。 acutaandM.资本充足率compressa的原核生物群落以单一细菌为主。组成方法（通过微生物平衡）可以识别微生物群落丰度的细微差异，通过所有宿主的漂白敏感性和时间来驱动变化。卡内奥赫湾的三个主要珊瑚礁创始物种在 2014-2015 年热浪之后表现出不同的表型和微生物组反应。很难预测针对未来全球变暖情景更成功的策略。随时间变化和/或白化敏感性不同的微生物类群在所有宿主之间广泛存在，这表明在当地，相同的微生物可能会调节同域珊瑚物种的应激反应。我们的研究强调了研究微生物平衡以识别精细微生物组变化的潜力，作为珊瑚礁健康状况的局部诊断工具。
Historically, Hawai‘i had few massive coral bleaching events, until two consecutive heatwaves in 2014–2015. Consequent mortality and thermal stress were observed in Kāne‘ohe Bay (O‘ahu). The two most dominant local species exhibited a phenotypic dichotomy of either bleaching resistance or susceptibility (Montipora capitataandPorites compressa), while the third predominant species (Pocillopora acuta) was broadly susceptible to bleaching. In order to survey shifts in coral microbiomes during bleaching and recovery, 50 colonies were tagged and periodically monitored. Metabarcoding of three genetic markers (16S rRNA gene ITS1 and ITS2) followed by compositional approaches for community structure analysis, differential abundance and correlations for longitudinal data were used to temporally compare Bacteria/Archaea, Fungi and Symbiodiniaceae dynamics.P. compressacorals recovered faster thanP. acutaandMontipora capitata. Prokaryotic and algal communities were majorly shaped by host species, and had no apparent pattern of temporal acclimatization. Symbiodiniaceae signatures were identified at the colony scale, and were often related to bleaching susceptibility. Bacterial compositions were practically constant between bleaching phenotypes, and more diverse inP. acutaandM. capitata.P. compressa's prokaryotic community was dominated by a single bacterium. Compositional approaches (via microbial balances) allowed the identification of fine-scale differences in the abundance of a consortium of microbes, driving changes by bleaching susceptibility and time across all hosts. The three major coral reef founder-species in Kāne‘ohe Bay revealed different phenotypic and microbiome responses after 2014–2015 heatwaves. It is difficult to forecast, a more successful strategy towards future scenarios of global warming. Differentially abundant microbial taxa across time and/or bleaching susceptibility were broadly shared among all hosts, suggesting that locally, the same microbes may modulate stress responses in sympatric coral species. Our study highlights the potential of investigating microbial balances to identify fine-scale microbiome changes, serving as local diagnostic tools of coral reef fitness.