Prenatal and lactational exposure to low-doses of bisphenol A alters adult mice behavior
Prenatal and lactational exposure to low-doses of bisphenol A alters adult mice behavior
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DOI：
10.1016/j.braindev.2010.12.011
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发表时间：
2012-01-01
影响因子：
1.7
通讯作者：
Fushiki, Shinji
中科院分区：
文献类型：
--
作者：
Nakamura, Keiko;Itoh, Kyoko;Fushiki, Shinji
双酚A （BPA）是一种干扰内分泌的化学物质，广泛用于牙科和各种行业。我们之前报道过BPA通过加速神经元分化/迁移影响小鼠新皮质发育，导致成年小鼠大脑新皮质结构异常以及丘脑皮质连接异常。本研究的目的是调查产前和哺乳期BPA暴露是否会影响成年小鼠的行为。从胚胎第0天（E0）至出生后第21天（P21），每天皮下注射20 μ g/kg BPA。对照动物单独得到一辆车。在产后3周（P3W）和P10-15W分别进行行为学测试（n = 15-20）。在野外测试后，进行了高架迷宫和Morris水迷宫测试。与对照组相比，bpa暴露组在P3W时的高架加迷宫试验和P10W时的露天试验的总距离显著减少。在P3W的空地试验中，在中心区域停留的时间和P11W的高架加迷宫试验中，在总距离上观察到显著的性别差异。这些结果表明，产前和哺乳期BPA暴露对小鼠产后发育和成年小鼠的行为有影响。(C) 2011日本儿童神经病学学会。Elsevier B.V.版权所有。
Bisphenol A (BPA) is an endocrine-disrupting chemical, widely used in dentistry and various industries. We previously reported that BPA affected murine neocortical development by accelerating neuronal differentiation/migration, resulting in abnormal neocortical architecture as well as aberrant thalamocortical connections in the brains of adult mice. The aim of this study was to investigate whether prenatal and lactational BPA exposure affected behavior in adult mice. Pregnant mice were injected subcutaneously with 20 mu g/kg of BPA daily from embryonic day 0 (E0) until postnatal day 21 (P21). Control animals received a vehicle alone. Behavioral tests (n = 15-20) were conducted at postnatal 3 weeks (P3W) and P10-15W. After an open-field test, an elevated plus maze and Morris water maze tests were performed. The total distance in the elevated plus maze test at P3W and in the open-field test at P10W was significantly decreased in the BPA-exposed group, compared with the control group. Significant sex differences were observed in the time spent in the central area in the open-field test at P3W and in the total distance in the elevated plus maze test at P11W. These results indicated that prenatal and lactational BPA exposure disturbed the murine behavior in the postnatal development period and the adult mice. (C) 2011 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.